Gurevitch O, Fabian I
Department of Histology and Cell Biology, Sackler School of Medicine, Tel Aviv University, Israel.
Stem Cells. 1993 Jan;11(1):56-61. doi: 10.1002/stem.5530110110.
A comparison of proliferative potential was made between spleen colony forming unit (CFU-S) from skeletal bones and those from ectopically induced ossicles. To this end, mice having two types of ectopic ossicles were used. Each animal was implanted s.c. with demineralized tooth matrix (which served as a source of bone-inducing activity) and again beneath the renal capsule with a femoral bone marrow plug (which was a source of viable osteogenic cells). Those recipient mice that had developed ectopic ossicles were lethally irradiated and then reconstituted with syngeneic bone marrow cells. At various times post-reconstitution, the proliferative potential (PP) of CFU-S in skeletal bones and ectopic ossicles of mice was studied in a double spleen colony test. Hemopoietic cells from each source were injected into lethally irradiated mice; in 11 days, spleen colonies were cut out, and their cells were reinjected into secondary irradiated recipients. The PP of CFU-S was evaluated by examining the development of daughter CFU-S. It was found that, in the hemopoietic microenvironment of induced ossicles, skeletal bone and ectopic ossicles developed by viable osteogenic cells, the PP of CFU-S is maintained to the same extent.
对来自骨骼的脾集落形成单位（CFU-S）和异位诱导骨化小体的脾集落形成单位的增殖潜力进行了比较。为此，使用了具有两种类型异位骨化小体的小鼠。每只动物皮下植入脱矿质牙基质（作为骨诱导活性的来源），并在肾被膜下再次植入股骨骨髓栓（作为有活力的成骨细胞的来源）。那些已形成异位骨化小体的受体小鼠接受致死性照射，然后用同基因骨髓细胞进行重建。在重建后的不同时间，通过双脾集落试验研究小鼠骨骼和异位骨化小体中CFU-S的增殖潜力（PP）。将来自每个来源的造血细胞注入致死性照射的小鼠体内；11天后，切下脾集落，将其细胞重新注入二次照射的受体小鼠体内。通过检查子代CFU-S的发育来评估CFU-S的PP。结果发现，在由有活力的成骨细胞形成的诱导骨化小体、骨骼和异位骨化小体的造血微环境中，CFU-S的PP保持在相同程度。
