Kurz S, Tiedtke A
Institute for General Zoology and Genetics, University of Münster, Germany.
J Eukaryot Microbiol. 1993 Jan-Feb;40(1):10-3. doi: 10.1111/j.1550-7408.1993.tb04874.x.
Electron microscopic investigations reveal that the Golgi apparatus of Tetrahymena thermophila consists of numerous tiny dictyosomes, each consisting of one or two cisternae. The dictyosomes are localized predominantly in the cell cortex closely associated with the mitochondria, arranged in meridians alternating with the ciliary meridians. We estimated about 300-400 of these dictyosomes in the periphery of a cell, a value corresponding to the number of somatic cilia per cell. Cytochemical assays of thiamine pyrophosphatase and acid phosphatase, both marker enzymes of trans Golgi cisternae, resulted in deposits of lead or cerium phosphate in the outermost cisternae of the dictyosomes. In addition, cisternae located at the bases of the basal body/parasomal sac arrangements are stained. This indicates that these cisternae may belong to the Golgi apparatus of the cell.
电子显微镜研究表明，嗜热四膜虫的高尔基体由众多微小的扁平囊泡组成，每个扁平囊泡由一或两个潴泡构成。这些扁平囊泡主要位于细胞皮层，与线粒体紧密相连，沿子午线排列，与纤毛子午线交替分布。我们估计在一个细胞的周边约有300 - 400个这样的扁平囊泡，这一数值与每个细胞的体纤毛数量相对应。硫胺素焦磷酸酶和酸性磷酸酶的细胞化学分析，这两种都是反式高尔基体潴泡的标记酶，导致在扁平囊泡最外层潴泡中出现磷酸铅或磷酸铈沉淀。此外，位于基体/副基体囊泡排列基部的潴泡也被染色。这表明这些潴泡可能属于细胞的高尔基体。
