Groff W A, Ellin R I, Skalsky R L
J Pharm Sci. 1977 Mar;66(3):389-91. doi: 10.1002/jps.2600660320.
A quantitative assay for measuring physostigmine in human whole blood is described. Blood samples are maintained at 37 degrees, and cholinesterase activity is measured periodically. The time required for enzyme reactivation is related to physostigmine concentration. The precision of the method is within +/- 4% over a physostigmine concentration range of 0.5-5.0 x 10(-7) M.
本文描述了一种用于测量人全血中毒扁豆碱的定量测定方法。血样保持在37摄氏度，并定期测量胆碱酯酶活性。酶重新激活所需的时间与毒扁豆碱浓度相关。该方法在0.5 - 5.0×10⁻⁷ M的毒扁豆碱浓度范围内的精密度在±4%以内。
