树眼镜蛇毒素阻断一类钾通道，这类钾通道在大鼠皮质突触体和脑片中由抑制性突触前调节剂开启。
Dendrotoxin blocks a class of potassium channels that are opened by inhibitory presynaptic modulators in rat cortical synaptosomes and slices.
作者信息
Zoltay G, Cooper J R
机构信息
Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06510.
出版信息
Cell Mol Neurobiol. 1993 Feb;13(1):59-68. doi: 10.1007/BF00712989.
Abstract
- Rat cortical synaptosomes were prelabeled with radioactive acetylcholine and the release induced by veratridine was determined in the absence and presence of the inhibitory presynaptic modulators, 2-chloroadenosine, carbamylcholine, clonidine, and morphine. All four agents inhibited the evoked release of acetylcholine and this inhibition was reversed by dendrotoxin. 2. Using perfused cortical slices and an extracellular K-sensitive electrode, all modulators again increased K efflux that was blocked by dendrotoxin. In contrast, glybenclamide and tetraethylammonium did not block the modulator-induced efflux.
摘要
- 用放射性乙酰胆碱对大鼠皮质突触体进行预标记，并在不存在和存在抑制性突触前调节剂（2-氯腺苷、氨甲酰胆碱、可乐定和吗啡）的情况下，测定藜芦碱诱导的释放。所有这四种药剂均抑制乙酰胆碱的诱发释放，且这种抑制作用可被树突毒素逆转。2. 使用灌流皮质切片和细胞外钾敏感电极，所有调节剂再次增加了钾外流，而这种外流被树突毒素阻断。相比之下，格列本脲和四乙铵并未阻断调节剂诱导的外流。
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