Zhanel G G, Kim S O, Davidson R J, Hoban D J, Nicolle L E
Department of Medicine, Faculty of Pharmacy, University of Manitoba, Winnipeg, Canada.
Chemotherapy. 1993 Mar-Apr;39(2):105-11. doi: 10.1159/000239110.
The effect of subinhibitory concentrations (1/8 x, 1/16 x, 1/32 x MIC) of ciprofloxacin or gentamicin on the adherence of Pseudomonas aeruginosa to Vero cells and voided uroepithelial cells was studied using two different assay methods. Strains studied included both reference strains and clinical strains, including mucoid and nonmucoid isolates. Sub-MICs of ciprofloxacin decreased adherence to Vero cells and voided uroepithelial cells in 3 of 4 ciprofloxacin-susceptible nonmucoid isolates but not in ciprofloxacin-resistant or mucoid isolates. Sub-MICs of gentamicin significantly reduced adherence in 5 of 7 nonmucoid strains but not in mucoid strains. Gentamicin effect on adherence did not correlate with susceptibility to ciprofloxacin. We conclude that ciprofloxacin susceptibility determines the effect of sub-MICs of ciprofloxacin on P. aeruginosa adherence to Vero cells and voided uroepithelial cells. In addition, both the Vero cell assay and voided uroepithelial cell assay demonstrated similar results.
使用两种不同的检测方法，研究了环丙沙星或庆大霉素的亚抑菌浓度（1/8倍、1/16倍、1/32倍MIC）对铜绿假单胞菌黏附于Vero细胞和脱落尿路上皮细胞的影响。所研究的菌株包括参考菌株和临床菌株，其中有黏液型和非黏液型分离株。环丙沙星的亚抑菌浓度使4株对环丙沙星敏感的非黏液型分离株中的3株对Vero细胞和脱落尿路上皮细胞的黏附减少，但对环丙沙星耐药或黏液型分离株没有影响。庆大霉素的亚抑菌浓度使7株非黏液型菌株中的5株黏附显著降低，但对黏液型菌株没有影响。庆大霉素对黏附的影响与对环丙沙星的敏感性无关。我们得出结论，环丙沙星敏感性决定了环丙沙星亚抑菌浓度对铜绿假单胞菌黏附于Vero细胞和脱落尿路上皮细胞的作用。此外，Vero细胞检测和脱落尿路上皮细胞检测都显示了相似的结果。
