Martinez de Ilarduya O, Vicente-Carbajosa J, Sanchez de la Hoz P, Carbonero P
Catedra de Bioquimica y Biologia Molecular, E.T.S. Ingenieros Agrónomos-UPM, Madrid, Spain.
FEBS Lett. 1993 Apr 5;320(2):177-81. doi: 10.1016/0014-5793(93)80087-b.
A cDNA of 2,708 bp encoding type 2 sucrose synthase (Ss2) from barley has been sequenced. Similarity of this cDNA with respect to that of type 1 (Ss1) is high in the coding region (75% identical positions), but low (41% identical residues) in the 3' non-coding region. Type-specific non cross-hybridizing probes for Northern blot analysis have been obtained from the 3' ends. The Ss1 type is highly expressed in developing endosperm and in roots and, at lower levels, in coleoptiles and aleurone. The Ss2 mRNA is abundant in endosperm, low in aleurone, and undetected in coleoptiles and roots.
已对来自大麦的编码2型蔗糖合酶（Ss2）的2708 bp cDNA进行了测序。该cDNA与1型（Ss1）的cDNA在编码区的相似性较高（75%的相同位置），但在3'非编码区的相似性较低（41%的相同残基）。已从3'端获得用于Northern印迹分析的类型特异性非交叉杂交探针。Ss1型在发育中的胚乳和根中高表达，在胚芽鞘和糊粉层中表达水平较低。Ss2 mRNA在胚乳中丰富，在糊粉层中含量低，在胚芽鞘和根中未检测到。
