Nikolaeva M A, Kulakov V I, Ter-Avanesov G V, Terekhina L N, Pshenichnikova T J, Sukhikh G T
Russian Scientific Research Center for Perinatology, Obstetrics and Gynaecology, Moscow.
Fertil Steril. 1993 Mar;59(3):639-44. doi: 10.1016/s0015-0282(16)55812-9.
To assess the use of flow cytometry (FCM) analysis of living spermatozoa subjected to indirect immunofluorescence staining with the mouse anti-human immunoglobulin (Ig)G monoclonal antibodies (FCM test) as an objective method for the detection of antisperm antibodies in semen and serum.
The comparative studies were conducted with the direct and indirect mixed antiglobulin reaction (MAR) and FCM test performed by FCM analyzer FACSCAN (Becton Dickinson Immunocytometry Systems, Mountain View, CA).
Clinical infertile service.
Seventy-six semen and 20 sera samples of infertile patients were analyzed.
For IgG in semen there was a highly significant correlation between the direct MAR and FCM test. Six of 12 IgG-positive samples revealed also IgA, and just one sample, which showed negative MAR, was positive for IgM in FCM test. Flow cytometry test and indirect MAR showed also a highly significant correlation of the results of IgG detection in sera.
Flow cytometry test may be used to verify that IgG antisperm antibodies are on the surface of living spermatozoa either retrieved directly from an ejaculate or after exposure to serum and to determine proportion of antisperm antibodies-positive spermatozoa, titer, and quantity of antibodies bound to the cell surface.
评估采用小鼠抗人免疫球蛋白（Ig）G单克隆抗体进行间接免疫荧光染色后，运用流式细胞术（FCM）分析活精子（FCM检测）作为检测精液和血清中抗精子抗体的一种客观方法的应用情况。
采用流式细胞分析仪FACSCAN（Becton Dickinson免疫细胞分析系统，加利福尼亚州山景城）进行直接和间接混合抗球蛋白反应（MAR）以及FCM检测，并开展对比研究。
临床不育诊疗服务机构。
对76份不育患者的精液样本和20份血清样本进行分析。
对于精液中的IgG，直接MAR与FCM检测之间存在高度显著相关性。12份IgG阳性样本中有6份同时显示IgA阳性，而在FCM检测中，仅有1份MAR结果为阴性的样本IgM呈阳性。流式细胞术检测与间接MAR在血清IgG检测结果方面也显示出高度显著相关性。
流式细胞术检测可用于验证IgG抗精子抗体是否存在于直接从射精样本中获取的活精子表面，或精子暴露于血清后的活精子表面，并确定抗精子抗体阳性精子的比例、滴度以及与细胞表面结合的抗体量。
