Summerbell R C
Ontario Ministry of Health, Toronto, Canada.
J Clin Microbiol. 1993 Mar;31(3):572-7. doi: 10.1128/jcm.31.3.572-577.1993.
The fungicide benomyl has long been known to differentially affect major taxonomic groups of fungi. In the present study 163 species or aggregates of closely similar species of medically important fungi and actinomycetes, as well as species commonly isolated as clinical contaminants, were tested to determine their reactions to three concentrations of benomyl. Fungi of basidiomycetous, endomycetous, and microascaceous affinities were highly resistant, including all common yeasts and Geotrichum, Pseudallescheria, Scedosporium, and Scopulariopsis species. Also resistant were fungi of pleosporalean affinities with poroconidial anamorphs, such as Alternaria, Bipolaris, Curvularia, and Exserohilum species. Most other fungi of ascomycetous affinity were moderately to strongly susceptible. Such fungi included dermatophytes; Coccidioides, Blastomyces, and Histoplasma species; Sporothrix schenckii; medically important aspergilli; and "black yeasts." Benomyl testing aided in the provisional identification of nonsporulating mycelia, including common basidiomycetous isolates obtained as contaminants as well as nonsporulating Aspergillus fumigatus from pulmonary sources.
长期以来，人们都知道杀菌剂苯菌灵对真菌的主要分类群有不同影响。在本研究中，对163种具有医学重要性的真菌和放线菌的物种或非常相似物种的集合，以及通常作为临床污染物分离出的物种进行了测试，以确定它们对三种浓度苯菌灵的反应。具有担子菌、内孢霉和小囊菌亲缘关系的真菌具有高度抗性，包括所有常见酵母以及地霉属、拟阿利什霉属、帚霉属和瓶霉属的物种。具有孔出分生孢子无性型的格孢腔菌亲缘关系的真菌也具有抗性，如链格孢属、离蠕孢属、弯孢属和突脐孢属的物种。大多数其他具有子囊菌亲缘关系的真菌中度至高度敏感。这类真菌包括皮肤癣菌；球孢子菌属、芽生菌属和组织胞浆菌属的物种；申克孢子丝菌；具有医学重要性的曲霉菌；以及“黑色酵母菌”。苯菌灵测试有助于对不产孢菌丝体进行初步鉴定，包括作为污染物获得的常见担子菌分离株以及来自肺部来源的不产孢烟曲霉。
