流式细胞术与静态图像细胞术在倍性测定中的比较。
Comparison of flow and static image cytometry in the determination of ploidy.
作者信息
Lanigan D, McLean P A, Curran B, Leader M
机构信息
Department of Urology, Beaumont Hospital, Ireland, Dublin, Eire.
出版信息
J Clin Pathol. 1993 Feb;46(2):135-9. doi: 10.1136/jcp.46.2.135.
AIMS
To compare the efficiency of flow cytometry and computed image cytometry; and to see if a reliable set of guidelines regarding interpretation of histograms could be drawn up.
METHODS
The two methods were applied to a series of 111 formalin fixed renal cell carcinomas. Data generated by both methods were compared.
RESULTS
The methods agreed in 85 cases. Hypodiploidy was detected by computed image cytometry in seven cases in which flow cytometry had shown only an aneuploid peak. Aneuploidy in seven in which the corresponding flow cytometry histogram was diploid. There was an overlap in the second peak proportions on flow cytometry histograms between those classed as diploid or tetraploid by computed image cytometry. In six cases the flow cytometry histograms had unacceptably high coefficients of variation and in all of these cases computed image cytometry demonstrated aneuploidy.
CONCLUSIONS
Computed image cytometry is particularly useful for clarifying difficult areas in flow histograms--specifically, high coefficients of variation, high G2M phase, as well as possible near diploid aneuploidy and hypodiploidy.
目的
比较流式细胞术和计算机图像细胞术的效率；并探讨是否能够制定出一套关于直方图解读的可靠指南。
方法
将这两种方法应用于111例福尔马林固定的肾细胞癌。对两种方法产生的数据进行比较。
结果
两种方法在85例中结果一致。在7例中，计算机图像细胞术检测到亚二倍体，而流式细胞术仅显示一个非整倍体峰。在7例中，相应的流式细胞术直方图为二倍体，但计算机图像细胞术检测到非整倍体。在计算机图像细胞术分类为二倍体或四倍体的样本中，流式细胞术直方图上第二个峰的比例存在重叠。在6例中，流式细胞术直方图的变异系数过高，在所有这些病例中，计算机图像细胞术均显示非整倍体。
结论
计算机图像细胞术对于澄清流式直方图中的疑难区域特别有用，特别是变异系数高、G2M期高，以及可能的近二倍体非整倍体和亚二倍体。
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