Michie B A, Black C, Reid R P, Barrett A, Hamblen D L
Musculo-skeletal Oncology Group, Western Infirmary, Glasgow, UK.
J Clin Pathol. 1994 May;47(5):443-7. doi: 10.1136/jcp.47.5.443.
To compare prognostic information obtained by image analysis cytometry of paraffin wax embedded soft tissue sarcomas with conventional assessment.
A CAS 200 image analyser was used to determine DNA content of Feulgen stained cytology preparations and tissue sections and to quantify immunostaining by Ki67 and PC10 antibodies. A mitotic count in 50 high power fields was undertaken and histological grade assigned by the Trojani system. Clinical details including follow up and outcome were obtained by case note review. The Kruskal-Wallis one way analysis test, Spearman rho significance test, Kaplan-Meier method, and log-rank test were applied in statistical analysis.
Ploidy status, DNA index, 2.5c exceeding rate, 5c exceeding rate, mitotic count and Trojani grade all correlated significantly with clinical outcome. The relation between Ki67 index and outcome did not reach significance. The PC10 index and outcome were not related. Only 2.5c exceeding rate, 5c exceeding rate, and mitotic count correlated significantly with Trojani grade.
DNA content determination of soft tissue sarcomas by image analysis provides quantifiable information of benefit in prediction of outcome. Larger series are required to determine the independent value of ploidy. In this study quantification of anti-Ki67 and anti-PC10 immunostaining was not of prognostic benefit) by contrast with mitotic count and Trojani grade.
比较石蜡包埋软组织肉瘤的图像分析细胞术所获得的预后信息与传统评估方法。
使用CAS 200图像分析仪测定福尔根染色细胞学标本和组织切片的DNA含量，并通过Ki67和PC10抗体对免疫染色进行定量分析。在50个高倍视野下进行有丝分裂计数，并根据特罗亚尼系统确定组织学分级。通过病例记录回顾获取包括随访和结果在内的临床详细信息。统计分析采用Kruskal-Wallis单向分析检验、Spearman等级相关性检验、Kaplan-Meier法和对数秩检验。
倍体状态、DNA指数、2.5c超标率、5c超标率、有丝分裂计数和特罗亚尼分级均与临床结果显著相关。Ki67指数与结果之间的关系未达到显著水平。PC10指数与结果无关。只有2.5c超标率、5c超标率和有丝分裂计数与特罗亚尼分级显著相关。
通过图像分析测定软组织肉瘤的DNA含量可提供对预后预测有益的可量化信息。需要更大规模的研究系列来确定倍体的独立价值。在本研究中，与有丝分裂计数和特罗亚尼分级相比，抗Ki67和抗PC10免疫染色的定量分析对预后无益处。
