Rainwater L M, Hosaka Y, Farrow G M, Lieber M M
J Urol. 1987 Jan;137(1):15-20. doi: 10.1016/s0022-5347(17)43857-2.
Nuclear deoxyribonucleic acid ploidy was determined in 206 samples of well differentiated clear cell renal carcinoma via a previously described technique on paraffin-embedded archival material. Grade 2 tumors had a higher incidence of abnormal deoxyribonucleic acid patterns than grade 1 tumors. Of stage 1 well differentiated clear cell renal tumors 60 per cent had a normal deoxyribonucleic acid histogram pattern, whereas 67 per cent of higher stage tumors had an abnormal pattern. The existence of abnormal nuclear deoxyribonucleic acid histogram patterns in the primary tumor tissue had a significant correlation with subsequent development of metastatic disease, independent of tumor grade and stage. The 10-year actuarial survival rate was 62 per cent for patients with normal deoxyribonucleic acid histograms and 37 per cent for patients with abnormal patterns. These results demonstrate that nuclear deoxyribonucleic acid ploidy measured by flow cytometry is an important variable in the classification and determination of prognosis for patients with clear cell renal carcinoma.
通过先前描述的技术，对206份石蜡包埋存档材料中的高分化透明细胞肾癌样本进行了细胞核脱氧核糖核酸倍性测定。2级肿瘤的脱氧核糖核酸模式异常发生率高于1级肿瘤。在1期高分化透明细胞肾肿瘤中，60%的肿瘤具有正常的脱氧核糖核酸直方图模式，而67%的更高分期肿瘤具有异常模式。原发性肿瘤组织中存在异常的细胞核脱氧核糖核酸直方图模式与转移性疾病的后续发展显著相关，与肿瘤分级和分期无关。脱氧核糖核酸直方图正常的患者10年精算生存率为62%，模式异常的患者为37%。这些结果表明，通过流式细胞术测量的细胞核脱氧核糖核酸倍性是透明细胞肾癌患者分类和预后判定的一个重要变量。
