Oskarsson H J, Godwin J, Gunnar R M, Thomas J X
Department of Medicine, Loyola University of Chicago, Stritch School of Medicine, Maywood, Illinois 60153.
J Am Coll Cardiol. 1993 Apr;21(5):1280-5. doi: 10.1016/0735-1097(93)90257-2.
This study was conducted to determine whether the long-term administration of fish oil attenuates myocardial necrosis in an occlusion-reperfusion model of myocardial ischemia.
Omega-3 fatty acids found in fish oil have various biologic properties that may modify myocardial injury caused by severe ischemia and reperfusion.
Of 21 dogs fed an identical diet, 10 were given supplemental fish oil containing 0.06 g/kg per day of eicosapentaenoic acid for 6 weeks. Under anesthesia and open chest conditions, the left circumflex coronary artery was occluded for 90 min, followed by 6 h of reperfusion. Regional myocardial blood flow was measured with 15-microns spheres before and during occlusion and during reperfusion. The area at risk and infarct size were measured using standard staining techniques.
In the dogs receiving supplemental fish oil, the platelet cell membrane content of eicosapentaenoic acid increased from 0.9 +/- 0.56% to 7.1 +/- 4.0% (p < 0.001). Infarct size was 29 +/- 7% in the control group and 13 +/- 3% in the fish oil group (p < 0.05). There was no significant difference in the myocardial area at risk or rate-pressure product between the control and fish oil groups. There was no difference in regional myocardial blood flow between the groups at baseline study or during coronary occlusion and reperfusion.
Dietary fish oil supplementation significantly reduced myocardial infarct size in this model. The difference in infarct size did not appear to be related to dissimilarities in regional myocardial blood flow or determinants of oxygen consumption. Further investigation is needed to determine the nature of the protective mechanisms of omega-3 fatty acids on myocardial infarct size.
本研究旨在确定长期给予鱼油是否能减轻心肌缺血再灌注模型中的心肌坏死。
鱼油中的ω-3脂肪酸具有多种生物学特性，可能会改变严重缺血和再灌注引起的心肌损伤。
在21只喂食相同饮食的犬中，10只犬每天补充含0.06 g/kg二十碳五烯酸的鱼油，持续6周。在麻醉和开胸条件下，左旋冠状动脉闭塞90分钟，随后再灌注6小时。在闭塞前、闭塞期间和再灌注期间，用15微米的微球测量局部心肌血流量。使用标准染色技术测量危险区域和梗死面积。
在接受补充鱼油的犬中，血小板细胞膜中二十碳五烯酸的含量从0.9±0.56%增加到7.1±4.0%（p<0.001）。对照组的梗死面积为29±7%，鱼油组为13±3%（p<0.05）。对照组和鱼油组之间的危险心肌面积或心率-血压乘积没有显著差异。在基线研究、冠状动脉闭塞和再灌注期间，两组之间的局部心肌血流量没有差异。
在该模型中，饮食补充鱼油可显著减小心肌梗死面积。梗死面积的差异似乎与局部心肌血流量或氧消耗决定因素的差异无关。需要进一步研究以确定ω-3脂肪酸对心肌梗死面积的保护机制的性质。
