Izuoka T, Kimura Y, Hamazaki T, Tamura T, Kitashiro S, Sugiura T, Jikuhara T, Iwasaka T
Second Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
Lipids. 1997 Jan;32(1):109-14. doi: 10.1007/s11745-997-0015-2.
To test the effects of eicosapentaenoic acid (EPA) infusion on pulmonary edema induced by coronary ligation and reperfusion, extravascular lung water (EVLW) was measured in situ by the thermal-dye double indicator dilution method in dogs. In the control group of five dogs, 30 mL of a 10% soybean oil emulsion was infused through a leg vein. One hour after infusion, the left anterior descending coronary artery below the first diagonal branch was ligated for 15 min and then reperfused for 30 min. In the EPA group, six dogs were similarly treated with an emulsion of a 10% trieicosapentaenoyl-glycerol (90% pure). EVLW, pulmonary capillary wedge pressure, mean pulmonary artery pressure, mean blood pressure, and cardiac index were measured before and 15 min after coronary ligation, and 15 min and 30 min after coronary reperfusion. There were no significant differences in the hemodynamic indices between the two groups. EVLW significantly increased up to two times of baseline during coronary ligation in the control group (P < 0.05) and more during reperfusion (P < 0.01), whereas EVLW did not increase in the EPA group. In conclusion, EPA inhibited EVLW accumulation and may be useful for ameliorating one of the ischemia-reperfusion-induced complications, pulmonary edema.
为了测试输注二十碳五烯酸（EPA）对冠状动脉结扎和再灌注诱导的肺水肿的影响，采用热染料双指示剂稀释法在犬原位测量血管外肺水（EVLW）。在5只犬的对照组中，经腿部静脉输注30 mL 10%的大豆油乳剂。输注1小时后，结扎第一对角支下方的左前降支冠状动脉15分钟，然后再灌注30分钟。在EPA组，6只犬接受类似处理，使用10%的甘油三亚二十碳五烯酸酯（90%纯）乳剂。在冠状动脉结扎前、结扎后15分钟、冠状动脉再灌注后15分钟和30分钟测量EVLW、肺毛细血管楔压、平均肺动脉压、平均血压和心脏指数。两组之间的血流动力学指标无显著差异。对照组在冠状动脉结扎期间EVLW显著增加至基线的两倍（P < 0.05），再灌注期间增加更多（P < 0.01），而EPA组的EVLW没有增加。总之，EPA抑制了EVLW的积聚，可能有助于改善缺血-再灌注诱导的并发症之一——肺水肿。
