Zhu B Q, Sievers R E, Sun Y P, Morse-Fisher N, Parmley W W, Wolfe C L
Department of Medicine, University of California, San Francisco.
Am Heart J. 1994 Apr;127(4 Pt 1):744-55. doi: 10.1016/0002-8703(94)90540-1.
Sprague-Dawley rats fed a diet containing 12% fish oil (18% eicosapentaenoic acid [EPA] and 12% docosahexaenoic acid [DHA]), for 1 week (group I, n = 9) or 8 weeks (group III, n = 42) and controls (group II, n = 8; group IV, n = 36, respectively) were subjected to 35 minutes of left coronary artery occlusion followed by 120 minutes of reperfusion. Compared to the controls, infarct size was significantly reduced in group III (15% +/- 2%, n = 42 vs 34% +/- 4%, n = 36; p < 0.001; infarct mass/risk area x 100%), but no change in group I (39% +/- 5%, n = 9 vs 35% +/- 5%, n = 8; p = not significant). Bleeding time was prolonged in group III (290 +/- 73 sec) compared to group IV (99 +/- 10 sec, p = 0.015). Omega-3 fatty acid (EPA and DHA) levels in platelets were significantly higher in the rats fed 8 weeks of fish oil (group III) compared to the controls (group IV) and the rats fed 8 weeks of fish oil and then a regular diet until bleeding time normalized (group V) (7.2% +/- 0.6% vs 1.2% +/- 0.2% and 4.9% +/- 0.5%; 3.8% +/- 0.7% vs 1.8% +/- 0.3% and 2.8% +/- 0.6%, p < 0.001 and 0.05, respectively). These data indicate that long-term (8 weeks) dietary fish oil supplementation significantly reduces infarct size; short-term (1 week) does not. This reduction of infarct size appears to correlate with altered platelet function and EPA and DHA levels in platelets.
给Sprague-Dawley大鼠喂食含12%鱼油（18%二十碳五烯酸[EPA]和12%二十二碳六烯酸[DHA]）的饲料1周（I组，n = 9）或8周（III组，n = 42），对照组（II组，n = 8；IV组，n = 36），然后对其进行35分钟的左冠状动脉闭塞，随后再灌注120分钟。与对照组相比，III组的梗死面积显著减小（15%±2%，n = 42 vs 34%±4%，n = 36；p < 0.001；梗死质量/危险区域×100%），但I组无变化（39%±5%，n = 9 vs 35%±5%，n = 8；p = 无显著性差异）。与IV组（99±10秒，p = 0.015）相比，III组的出血时间延长（290±73秒）。与对照组（IV组）以及喂食8周鱼油然后改为常规饮食直至出血时间恢复正常的大鼠（V组）相比，喂食8周鱼油的大鼠（III组）血小板中的ω-3脂肪酸（EPA和DHA）水平显著更高（7.2%±0.6% vs 1.2%±0.2%和4.9%±0.5%；3.8%±0.7% vs 1.8%±0.3%和2.8%±0.6%，p分别< 0.001和0.05）。这些数据表明，长期（8周）补充膳食鱼油可显著减小梗死面积；短期（1周）则无此效果。梗死面积的减小似乎与血小板功能改变以及血小板中EPA和DHA水平有关。
