Koopman G, Heider K H, Horst E, Adolf G R, van den Berg F, Ponta H, Herrlich P, Pals S T
Department of Pathology, Academic Medical Center, University of Amsterdam, The Netherlands.
J Exp Med. 1993 Apr 1;177(4):897-904. doi: 10.1084/jem.177.4.897.
A recently described splice variant of CD44 expressed in metastasizing cell lines of rat tumors, has been shown to confer metastatic potential to nonmetastasizing rat pancreatic carcinoma and sarcoma cell lines. Using antibodies raised against a bacterial fusion protein encoded by variant CD44 sequences, we have explored the expression of variant CD44 glycoproteins on human lymphoid cells and tissues and on non-Hodgkin's lymphomas. Normal lymphohematopoietic cells express barely detectable low levels of variant CD44 glycoproteins, whereas T lymphocytes, upon activation by mitogen or antigen, transiently upregulate expression of specific CD44 variant glycoproteins. The reaction pattern of various antibodies indicates that these CD44 variants contain the domain encoded by exon v6, which is part of the variant that in the rat confers metastatic capability. It is interesting that overexpression of v6 was also found in several aggressive, but not low-grade, non-Hodgkin's lymphomas.
最近在大鼠肿瘤转移细胞系中发现的一种CD44剪接变体，已被证明能赋予非转移性大鼠胰腺癌和肉瘤细胞系转移潜能。利用针对由CD44变体序列编码的细菌融合蛋白产生的抗体，我们研究了变体CD44糖蛋白在人淋巴细胞、组织以及非霍奇金淋巴瘤上的表达。正常淋巴造血细胞仅表达低水平、几乎检测不到的变体CD44糖蛋白，而T淋巴细胞在被有丝分裂原或抗原激活后，会短暂上调特定CD44变体糖蛋白的表达。多种抗体的反应模式表明，这些CD44变体包含由外显子v6编码的结构域，该外显子是大鼠中赋予转移能力的变体的一部分。有趣的是，在几种侵袭性而非低级别非霍奇金淋巴瘤中也发现了v6的过表达。
