Shinozuka H, Estes L W
J Natl Cancer Inst. 1977 Apr;58(4):1163-6. doi: 10.1093/jnci/58.4.1163.
Livers of 6- to 7-week-old male C3H/He, CBA, A, and BALB/c mice were examined by electron microscopy for the presence of intracisternal A particles (ICAP) after administration of diethylnitrosamine (DEN) in drinking water. In control mice, ICAP were extremely rare; they were found in the livers of only 2 mice (strains C3H/He and A; none in the other strains). By contrast, the treatment of mice with DEN greatly enhanced the appearance of ICAP in the liver cells of all strains. Within 2 weeks of the treatment, ICAP were found in 8-26% of liver cells examined in all mice and the number of ICAP/cell ranged from 3 to 12. Aside from mild disorganization of the rough endoplasmic reticulum, such as segmentation and vesiculation, liver cells of carcinogen-treated mice showed none of the consistent abnormalities that characterize the appearance of ICAP. The reactivation of ICAP (which are usually suppressed in adult mice) by DEN may become a useful marker for analysis of the sequential alterations of the liver that lead to the development of hepatoma during carcinogenesis.
给6至7周龄的雄性C3H/He、CBA、A和BALB/c小鼠饮用含二乙基亚硝胺（DEN）的水后，通过电子显微镜检查其肝脏中是否存在核内池A颗粒（ICAP）。在对照小鼠中，ICAP极其罕见；仅在2只小鼠（C3H/He和A品系；其他品系均未发现）的肝脏中发现了ICAP。相比之下，用DEN处理小鼠极大地增加了所有品系小鼠肝细胞中ICAP的出现率。处理后2周内，在所有小鼠检查的肝细胞中，8%至26%发现了ICAP，每个细胞中ICAP的数量为3至12个。除了粗面内质网有轻度的紊乱，如片段化和小泡化外，经致癌物处理的小鼠肝细胞未表现出任何与ICAP出现相关的一致异常特征。DEN使ICAP（通常在成年小鼠中受到抑制）重新激活，这可能成为分析致癌过程中导致肝癌发生的肝脏序列改变的有用标志物。
