Pollack H, Zhan M X, Ilmet-Moore T, Ajuang-Simbiri K, Krasinski K, Borkowsky W
Department of Pediatrics, New York University Medical Center, NY.
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2340-4. doi: 10.1073/pnas.90.6.2340.
The early serologic response of infants to infection with human immunodeficiency virus type 1 (HIV-1) is normally obscured by the presence of transplacentally acquired maternal HIV antibody. By measuring HIV antibody produced in vitro by lymphocytes isolated from peripheral blood of infants and children of HIV-1-infected mothers, we have been able to study the natural acquisition of humoral immunity to perinatal HIV-1 infection. One hundred ninety-seven infants of HIV-1-infected women were studied prospectively and longitudinally from birth. In the neonatal period, infected infants produced only small amounts of HIV-specific IgG antibodies to a restricted number of antigens. The amount of immunoglobulin to HIV-1 and the number of HIV-1 antigens recognized increased with age. After 6 months of life 85% of infected infants made detectable antibody to two or more viral proteins. Antibody to gp160 appeared first and was the most frequently found at all ages, followed by antibody to the envelope proteins gp120 and gp41. The amount of HIV antibody produced correlated positively with the percentage of CD4+ T lymphocytes in peripheral blood. This assay provides a method of studying the immunogenicity of vaccines against HIV-1 in HIV-1-infected infants and of assessing the effect of early therapeutic interventions on the humoral response to HIV-1.
婴儿对1型人类免疫缺陷病毒（HIV-1）感染的早期血清学反应通常会被经胎盘获得的母体HIV抗体所掩盖。通过检测从感染HIV-1母亲的婴儿和儿童外周血中分离出的淋巴细胞在体外产生的HIV抗体，我们得以研究对围产期HIV-1感染的体液免疫的自然获得情况。对197名感染HIV-1女性的婴儿从出生起进行了前瞻性和纵向研究。在新生儿期，受感染婴儿仅产生少量针对有限数量抗原的HIV特异性IgG抗体。针对HIV-1的免疫球蛋白量以及所识别的HIV-1抗原数量随年龄增加。6个月大后，85%的受感染婴儿产生了可检测到的针对两种或更多种病毒蛋白的抗体。针对gp160的抗体首先出现，且在所有年龄段中最常被检测到，其次是针对包膜蛋白gp120和gp41的抗体。产生的HIV抗体量与外周血中CD4+ T淋巴细胞百分比呈正相关。该检测方法为研究针对HIV-1感染婴儿的HIV-1疫苗的免疫原性以及评估早期治疗干预对HIV-1体液反应的影响提供了一种方法。
