Gómez-Pinilla F, Cotman C W
Department of Psychobiology, University of California, Irvine 92717.
Brain Res. 1993 Mar 19;606(1):79-86. doi: 10.1016/0006-8993(93)91572-a.
Fibroblast growth factors (FGFs) are potent growth factors with roles in the brain ranging from development to adult plasticity. FGF-5 is a newly described member of the fibroblast growth factor family. In order to evaluate a possible role of FGF-5, we have examined the locus of synthesis of FGF-5 in the rat brain. We have used in situ hybridization of 35S-labelled RNA probe complementary to FGF-5 mRNA. FGF-5 mRNA was present in neurons in select regions of the rat brain. FGF-5 mRNA expression was particularly intense in the olfactory bulb within periglomerular elements and the mitral cell layer. The primary olfactory cortex also showed a robust expression of FGF-5 mRNA mostly within layer II. In the hippocampal formation, the greatest labelling of FGF-5 mRNA occurred in hippocampal pyramidal cells within subfields CA3 and secondarily within subfields CA1, CA2 and CA4. The dentate gyrus granule cells displayed a modest hybridization signal. The cerebral cortex (neocortex) showed a light labelling throughout its rostro-caudal extent mostly within external layers. The entorhinal cortex showed a higher FGF-5 mRNA expression as compared to the neocortex and signal appeared more intense in layer II. In general, FGF-5 mRNA was shown to be localized mostly in limbic structures, suggesting that FGF-5 may play a role in limbic system function or dysfunction.
成纤维细胞生长因子（FGFs）是一类强大的生长因子，在大脑中发挥着从发育到成年可塑性的多种作用。FGF - 5是成纤维细胞生长因子家族新发现的成员。为了评估FGF - 5的潜在作用，我们检测了大鼠脑中FGF - 5的合成位点。我们使用了与FGF - 5 mRNA互补的35S标记RNA探针进行原位杂交。FGF - 5 mRNA存在于大鼠脑的特定区域的神经元中。FGF - 5 mRNA表达在嗅球的小球周元件和僧帽细胞层中尤为强烈。初级嗅觉皮层在II层内也显示出FGF - 5 mRNA的强烈表达。在海马结构中，FGF - 5 mRNA标记最强的是CA3亚区的海马锥体细胞，其次是CA1、CA2和CA4亚区。齿状回颗粒细胞显示出适度的杂交信号。大脑皮层（新皮层）在其整个前后范围内主要在外层显示出轻微的标记。与新皮层相比，内嗅皮层显示出更高的FGF - 5 mRNA表达，且在II层信号更强。总体而言，FGF - 5 mRNA主要定位于边缘结构，这表明FGF - 5可能在边缘系统功能或功能障碍中发挥作用。
