大鼠脑中成纤维细胞生长因子-9信使核糖核酸的定位

Localization of fibroblast growth factor-9 mRNA in the rat brain.

作者信息

Tagashira S, Ozaki K, Ohta M, Itoh N

机构信息

Department of Genetic Biochemistry, Kyoto University, Faculty of Pharmaceutical Sciences, Japan.

出版信息

Brain Res Mol Brain Res. 1995 Jun;30(2):233-41. doi: 10.1016/0169-328x(95)00009-h.

DOI:10.1016/0169-328x(95)00009-h

PMID:7637574

Abstract

We examined the localization of fibroblast growth factor-9 (FGF-9) mRNA in the rat brain by in situ hybridization. FGF-9 mRNA was moderately or weakly expressed in widespread regions including the olfactory bulb, caudate putamen, cerebral cortex, hippocampus, thalamus, hypothalamus, midbrain, brainstem and cerebellum. However, FGF-9 mRNA was also strongly expressed in several specific nuclei including the red nucleus and oculomotor nucleus in the midbrain, the vestibular nucleus and facial nucleus in the brainstem and the medial cerebellar nucleus, interposed cerebellar nucleus and lateral cerebellar nucleus in the cerebellum. The cellular localization of FGF-9 mRNA indicated that the mRNA in the rat brain was expressed preferentially in neurons, although FGF-9 was originally isolated from human glioma cells. The localization profile of FGF-9 mRNA is different from those of aFGF, bFGF and FGF-5 mRNAs reported previously. The present findings indicate that FGF-9 has a unique role in the brain.

摘要

我们通过原位杂交技术检测了大鼠脑中成纤维细胞生长因子9（FGF - 9）mRNA的定位。FGF - 9 mRNA在广泛区域呈中度或弱表达，包括嗅球、尾状壳核、大脑皮层、海马体、丘脑、下丘脑、中脑、脑干和小脑。然而，FGF - 9 mRNA在几个特定核团中也强烈表达，包括中脑的红核和动眼神经核、脑干的前庭核和面神经核以及小脑的小脑内侧核、间位小脑核和小脑外侧核。FGF - 9 mRNA的细胞定位表明，尽管FGF - 9最初是从人胶质瘤细胞中分离出来的，但大鼠脑中的mRNA优先在神经元中表达。FGF - 9 mRNA的定位图谱与先前报道的aFGF、bFGF和FGF - 5 mRNA的定位图谱不同。目前的研究结果表明FGF - 9在脑中具有独特作用。