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班氏吴策线虫微丝蚴中人类血清所识别的表面和细胞内抗原的免疫细胞化学定位

Immunocytochemical localization of surface and intracellular antigens recognized by human sera in microfilariae of Wuchereria bancrofti.

作者信息

Souto-Padrón T, Dreyer G, Andrade L D, Rocha A, De Souza W

机构信息

Laboratory of Cellular Ultrastructure, Institute of Biophysics Carlos Chagas Filho, Federal University, Rio de Janeiro, Brasil.

出版信息

J Submicrosc Cytol Pathol. 1993 Jan;25(1):79-83.

PMID:8462071
Abstract

Sera from patients with various clinical pictures of lymphatic filariasis, including tropical pulmonary eosinophilia (TPE), were used for the localization of surface and intracellular antigens in microfilariae of Wuchereria bancrofti embedded in Lowicryl K4M. Very few or no antigenic sites were located on the outer face of the sheath. The most inner layer, as well as the space between the cuticle and the sheath, was intensely labeled. Sera from TPE patients intensely labeled the cuticle and the cytoplasm of muscle cells.

摘要

来自患有各种淋巴丝虫病临床症状的患者的血清,包括热带肺嗜酸性粒细胞增多症(TPE),用于定位包埋在Lowicryl K4M中的班氏吴策线虫微丝蚴表面和细胞内抗原。在鞘的外表面很少或没有发现抗原位点。最内层以及角质层和鞘之间的空间被强烈标记。TPE患者的血清强烈标记了角质层和肌肉细胞的细胞质。

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