Souto-Padrón T, Dreyer G, De Andrade L D, De Souza W
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
J Submicrosc Cytol Pathol. 1990 Jan;22(1):47-52.
Antigenic sites were localized on the surface structure (sheath and cuticle) of microfilariae of Wuchereria bancrofti using sera from microfilaraemic and amicrofilaraemic patients using antibodies associated to colloidal gold particles and transmission electron microscopy. No labeling was observed on the surface of intact parasites. Microfilariae without the sheath and isolated sheath were obtained by ultrasound treatment. Using these preparations labeling of the inner portion of the sheath was observed when sera from amicrofilaraemic patients were used. Labeling of the cuticle surface was observed mainly in the regions of the cuticular annulations.
利用来自微丝蚴血症患者和无微丝蚴血症患者的血清，借助与胶体金颗粒相关的抗体及透射电子显微镜，将班氏吴策线虫微丝蚴的抗原位点定位在其表面结构（鞘膜和角质层）上。在完整寄生虫的表面未观察到标记。通过超声处理获得了无鞘膜的微丝蚴和分离的鞘膜。使用这些制剂，当使用无微丝蚴血症患者的血清时，观察到鞘膜内部有标记。角质层表面的标记主要出现在角质层环纹区域。
