Klein R L, Lopes-Virella M F
Research Service, Ralph H. Johnson Department of Veteran Affairs Medical Center, Charleston, South Carolina.
Diabetologia. 1993 Mar;36(3):258-64. doi: 10.1007/BF00399960.
The very low density lipoprotein (VLDL) fraction was isolated from 11 normolipidaemic Type 1 (insulin-dependent) diabetic patients in good to fair glycaemic control and from 11 age-, sex- and race-matched, non-diabetic, control subjects. The rate of receptor-mediated degradation by human endothelial cells was significantly greater (p < 0.02) for the total VLDL fraction isolated from diabetic patients compared to control subjects and averaged 1008 +/- 300 and 717 +/- 150 ng.mg cell protein-1.16 h-1, respectively. The total VLDL fraction was separated into three subfractions: VLDL-I, Sf 100-400 (Sf = Svedberg units); VLDL-II, Sf 60-100; VLDL-III, Sf 20-60. Rates of receptor-mediated degradation of VLDL-I and VLDL-II isolated from diabetic patients were significantly greater than the comparable subfraction isolated from control subjects and averaged 1023 +/- 279 vs 361 +/- 122 (p < 0.01) and 433 +/- 70 vs 294 +/- 70 ng.mg cell protein-1.16 h-1 (p < 0.03), respectively. Rates of receptor-mediated degradation of the V-III subfraction isolated from the two groups did not differ significantly. There were no significant differences in the chemical composition or in the plasma concentrations of the VLDL subfractions isolated from diabetic patients compared to control subjects. There was a significant increase in the apoprotein E content of VLDL-I (p < 0.01) and VLDL-II (p < 0.05) isolated from diabetic patients. There was a significant increase in the ratio of apoprotein C compared to apoprotein E (p < 0.03) in VLDL-I isolated from control subjects compared to the diabetic patients.(ABSTRACT TRUNCATED AT 250 WORDS)
从11名血糖控制良好至尚可的血脂正常的1型（胰岛素依赖型）糖尿病患者以及11名年龄、性别和种族匹配的非糖尿病对照受试者中分离出极低密度脂蛋白（VLDL）组分。与对照受试者相比，从糖尿病患者分离出的总VLDL组分被人内皮细胞受体介导的降解速率显著更高（p<0.02），分别平均为1008±300和717±150 ng·mg细胞蛋白⁻¹·16 h⁻¹。总VLDL组分被分离为三个亚组分：VLDL-I，Sf 100 - 400（Sf = 斯维德伯格单位）；VLDL-II，Sf 60 - 100；VLDL-III，Sf 20 - 60。从糖尿病患者分离出的VLDL-I和VLDL-II的受体介导降解速率显著高于从对照受试者分离出的相应亚组分，分别平均为1023±279对361±122（p<0.01）和433±70对294±70 ng·mg细胞蛋白⁻¹·16 h⁻¹（p<0.03）。从两组分离出的V-III亚组分的受体介导降解速率无显著差异。与对照受试者相比，从糖尿病患者分离出的VLDL亚组分在化学组成或血浆浓度方面无显著差异。从糖尿病患者分离出的VLDL-I（p<0.01）和VLDL-II（p<0.05）的载脂蛋白E含量显著增加。与糖尿病患者相比，从对照受试者分离出的VLDL-I中载脂蛋白C与载脂蛋白E的比率显著增加（p<0.03）。（摘要截短于250字）
