Wahle K W, Radcliffe J D
Lipids. 1977 Feb;12(2):135-9. doi: 10.1007/BF02533282.
Aspects of the lipid metabolism of male, obese and lean Zucker rats were compared using animals which had been fed ad libitum for 32 days on a diet (HS) which contained 200 g sunflowerseed oil/kg or one (LS) which contained 50 g/kg of the oil. When compared with the LS diet, the HS diet decreased the characteristic lipid accretion in the liver of obses rats from 126 mg (LS) to 81 mg (HS)/g wet weight; corresponding values for the lean rats were 39 mg and 56 mg/g wet weight of liver, respectively. The HS diet depressed lipid synthesis de novo by liver homogenates and decreased the delta9-desaturase activity of liver microsomes from obese and lean rats by about 50%. delta9-Desaturase activity in vitro was also depressed by the addition of linoleic acid to liver microsomes from both obese and lean rats fed ad libitum on a standard laboratory diet. Depressed delta9-desaturase activity, due to ingestion of the HS diet, was reflected in lower ratios of 16:1/16:0 and 18:1/18:0 fatty acids in tissue lipids from obese and lean rats. Ingestion of the HS compared with the LS diet resulted in increased proportions of 18:2omega6 in liver lipids and adipose tissue triacylglycerols of obese and lean rats. The HS diet also increased the proportions of 20:4omega6 in adipose triacylglycerols of obese and lean rats and in liver lipids of obese animals but not in their lean littermates.
使用雄性肥胖和瘦型 Zucker 大鼠，比较它们的脂质代谢情况。这些大鼠被随意喂食 32 天，一种饮食（HS）含有 200 g 葵花籽油 /kg，另一种饮食（LS）含有 50 g/kg 的该种油。与 LS 饮食相比，HS 饮食使肥胖大鼠肝脏中特征性脂质蓄积量从 126 mg（LS）降至 81 mg（HS）/g 湿重；瘦型大鼠相应的值分别为 39 mg 和 56 mg/g 肝脏湿重。HS 饮食抑制了肝脏匀浆中从头合成脂质的过程，并使肥胖和瘦型大鼠肝脏微粒体的 Δ9- 去饱和酶活性降低了约 50%。对于随意喂食标准实验室饮食的肥胖和瘦型大鼠，向其肝脏微粒体中添加亚油酸也会抑制体外 Δ9- 去饱和酶活性。由于摄入 HS 饮食导致的 Δ9- 去饱和酶活性降低，反映在肥胖和瘦型大鼠组织脂质中 16:1/16:0 和 18:1/18:0 脂肪酸比例较低。与 LS 饮食相比，摄入 HS 饮食导致肥胖和瘦型大鼠肝脏脂质和脂肪组织三酰甘油中 18:2ω6 的比例增加。HS 饮食还增加了肥胖和瘦型大鼠脂肪三酰甘油以及肥胖动物肝脏脂质中 20:4ω6 的比例，但在它们瘦型同窝仔鼠的肝脏脂质中未增加。
