Body D R
Lipids. 1977 Feb;12(2):204-7. doi: 10.1007/BF02533295.
The isoprenoid hydrocarbon, trans-phyt-2-ene (82.2%), phyt-1-ene (6.5%), and phytane (11.3%), were detected in bovine rumen liquor by means of AgNO3-impregnated thin layer chromatography and gas liquid chromatography with mass spectrometry and infrared spectrophotometry. The unsaturated hydrocarbons were identified by comparing their chromatographic, physical, and chemical properties with those of synthetic isoprenoid hydrocarbons prepared from dihydrophytol. The natural level of the original isoprenoid components within the rumen is comprised of phytol and dihydrophytol (of the ratio of 2:1). These represented at least 60% of the total free fatty alcohol content of the rumen liquor lipid extract and could, therefore, be the origin of the isoprenoid hydrocarbons observed.
通过硝酸银浸渍薄层色谱法、气相色谱-质谱联用以及红外分光光度法，在牛瘤胃液中检测到了类异戊二烯烃反式植-2-烯（82.2%）、植-1-烯（6.5%）和植烷（11.3%）。通过将这些不饱和烃的色谱、物理和化学性质与由二氢植醇制备的合成类异戊二烯烃的性质进行比较，对其进行了鉴定。瘤胃中原始类异戊二烯成分的天然水平由植醇和二氢植醇（比例为2:1）组成。这些成分至少占瘤胃液脂质提取物中总游离脂肪醇含量的60%，因此可能是所观察到的类异戊二烯烃的来源。
