Ebisuno S, Miyata K, Kohjimoto Y, Ohkawa T
Department of Urology, Minami Wakayama National Hospital.
Nihon Hinyokika Gakkai Zasshi. 1993 Feb;84(2):339-44. doi: 10.5980/jpnjurol1989.84.339.
We studied inhibitory activity of urinary macromolecule upon the calcium oxalate crystal aggregation using an aggregometer. We have developed an aggregometric assay method to measure the anti-aggregation ratio of calcium oxalate crystals in vitro. The macromolecular fraction of urine with a molecular weight above 5,000 was isolated by PD-10 (Sephadex G-25M, Pharmacia) and made up to three-fold by Centriprep Concentrator (Amicon). The urinary macromolecular substances of recurrent calcium stone formers showed much less inhibitory activity than those of healthy controls. There were no significant relationships between the anti-aggregation activity and the concentration of urinary proteins and other parameters concerning with stone diseases. It was speculated that some proteins, molecular weight about 10,000-30,000, might inhibit the aggregation vigorously based on the result of gel filtration (Superrose 12 HR, 20/50, Pharmacia) technique used in a healthy man's 24 hours urine. Thus, it supports that some urinary macromolecules are important during the phase of aggregation of calcium oxalate crystals, and that the feeble activity to present the aggregation may be one of the cause of calcium oxalate stone formation.
我们使用凝集仪研究了尿大分子对草酸钙晶体聚集的抑制活性。我们开发了一种凝集测定法来测量体外草酸钙晶体的抗聚集率。通过PD - 10（葡聚糖凝胶G - 25M，Pharmacia）分离出分子量大于5000的尿大分子部分，并通过Centriprep浓缩器（Amicon）浓缩至三倍。复发性草酸钙结石形成者的尿大分子物质的抑制活性远低于健康对照者。抗聚集活性与尿蛋白浓度及其他与结石疾病相关的参数之间无显著相关性。根据对一名健康男性24小时尿液使用凝胶过滤（Superose 12 HR，20/50，Pharmacia）技术的结果推测，一些分子量约为10000 - 30000的蛋白质可能强烈抑制聚集。因此，这支持了一些尿大分子在草酸钙晶体聚集阶段很重要，而其抑制聚集的活性较弱可能是草酸钙结石形成的原因之一。
