Ebisuno S, Kohjimoto Y, Yoshida T, Ohkawa T
Department of Urology, Minami Wakayama National Hospital, Tanabe shi, Japan.
Urol Res. 1993;21(4):265-8. doi: 10.1007/BF00307708.
The inhibitory activity of urinary macromolecules on the aggregation of calcium oxalate crystals was studied using an aggregometer originally devised to measure thrombocyte aggregation capacity by means of the optical turbidity at 660 nm. The macromolecular fraction of the urine (molecular weight above 5000) of recurrent calcium stone formers showed much less inhibitory activity than that of healthy controls (P < 0.05). It was speculated on the basis of the results of gel filtration that there were some proteins (molecular weight about 10,000-30,000) which had inhibitory activities for the aggregation of calcium oxalate. This gives support to the assumption that macromolecules are important during the phase of aggregation of calcium oxalate crystals.
使用最初设计用于通过测量660nm处的光学浊度来测定血小板聚集能力的凝集仪，研究了尿中大分子对草酸钙晶体聚集的抑制活性。复发性钙结石形成者尿液中的大分子部分（分子量高于5000）显示出比健康对照少得多的抑制活性（P<0.05）。根据凝胶过滤结果推测，存在一些对草酸钙聚集具有抑制活性的蛋白质（分子量约为10,000-30,000）。这支持了大分子在草酸钙晶体聚集阶段很重要的假设。
