Ceberg C P, Salford L G, Brun A, Hemler R J, Persson B R
Department of Radiation Physics, University Hospital, Lund, Sweden.
Radiother Oncol. 1993 Feb;26(2):139-46. doi: 10.1016/0167-8140(93)90095-p.
Boron Neutron Capture Therapy (BNCT) is an attractive concept for radiation treatment of malignant tumours. The patients receive a 10B-carrying compound with selective uptake in tumour cells, after which they are irradiated with epithermal neutrons. Theoretically, the tumour cells are killed by the high-LET particles produces in 10B(n, alpha)7Li reactions inside or close to the cell nucleus, while healthy brain cells with no boron uptake will be spared. In practice, a successful BNCT depends on the actual boron-distribution in the tissue, and consequently a new boron-compound aimed for BNCT must undergo detailed bio-distribution studies before clinical trials. In experimental work there is accordingly a great need for methods for quantitative bio-distribution measurements in tissue samples. In this paper we present an improved technique for neutron activated autoradiography providing quantitative boron images of freeze-sectioned tissue specimens from highly malignant rat brain gliomas. Particular attention has been paid to the correlation with the morphology of the specimens and to the altered self-absorption properties due to freeze-drying. A self-absorption correction factor for tumour tissue has been experimentally determined.
硼中子俘获疗法（BNCT）是一种用于恶性肿瘤放射治疗的极具吸引力的概念。患者先接受一种能被肿瘤细胞选择性摄取的含硼-10化合物，之后用超热中子进行照射。理论上，肿瘤细胞会被在细胞核内或细胞核附近的硼-10（n，α）7Li反应中产生的高传能线密度粒子杀死，而未摄取硼的健康脑细胞则可幸免。实际上，成功的BNCT取决于组织中硼的实际分布情况，因此，一种用于BNCT的新型硼化合物在进行临床试验之前必须进行详细的生物分布研究。在实验工作中，因此非常需要用于组织样本中定量生物分布测量的方法。在本文中，我们提出了一种改进的中子活化放射自显影技术，可提供来自高度恶性大鼠脑胶质瘤的冷冻切片组织标本的定量硼图像。特别关注了与标本形态的相关性以及冷冻干燥导致的自吸收特性改变。已通过实验确定了肿瘤组织的自吸收校正因子。
