Slama J T, Simmons A M, Hernandez T M, Keenan R W
Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284-7760.
Anal Biochem. 1993 Feb 15;209(1):143-9. doi: 10.1006/abio.1993.1094.
Elemental [35S]sulfur was shown to equilibrate with the sulfur of thiophosphoryl chloride when these materials are heated together. This isotopic exchange reaction is the basis of a convenient, microscale synthesis of high specific activity [35S]PSCl3. [35S]Thiophosphoryl chloride is otherwise not commercially available except through custom synthesis. The labeled thiophosphoryl chloride was used in a novel procedure for the preparation of [35S]adenosine 5'-phosphorothioate. This isotopic exchange method should find wide application in the synthesis of many radiolabeled thiophosphoryl esters which utilize PSCl3 as the source of the thiophosphoryl group.
