Yang F, Jansen L, Friedrichs W E, Buchanan J M, Bowman B H
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762.
Biochem Biophys Res Commun. 1993 Mar 31;191(3):1014-9. doi: 10.1006/bbrc.1993.1318.
In Alzheimer's disease a small fragment of the amyloid protein precursor (APP), called beta 4, is a characteristic component of senile plaques in brains of affected patients. Efforts to intervene in Alzheimer's disease include approaches by which APP levels can be decreased in brain. The study described here demonstrates the expression of APP gene in four cell lines that originated from human brain glioblastomas. In one line, HTB 17, APP mRNA level was approximately 25% the APP mRNA found in human brain and 150% that found in human liver. To ascertain whether or not APP expression in HTB 17 cells could be modulated by a cytokine associated with the inflammatory response, cells were cultured in the presence of IL-1 beta. A significant decrease in APP mRNA accompanied treatment of glioma cells with IL-1 beta.
在阿尔茨海默病中，淀粉样蛋白前体（APP）的一个小片段，称为β4，是受影响患者大脑中老年斑的特征性成分。干预阿尔茨海默病的努力包括降低大脑中APP水平的方法。本文所述的研究证明了APP基因在源自人脑胶质母细胞瘤的四种细胞系中的表达。在一种细胞系HTB 17中，APP mRNA水平约为人脑APP mRNA的25%，是人类肝脏中APP mRNA的150%。为了确定HTB 17细胞中的APP表达是否可以被与炎症反应相关的细胞因子调节，细胞在IL-1β存在的情况下进行培养。用IL-1β处理胶质瘤细胞后，APP mRNA显著下降。
