Winkles J A, Alberts G F, Brogi E, Libby P
Department of Molecular Biology, Holland Laboratory, American Red Cross, Rockville, MD 20855.
Biochem Biophys Res Commun. 1993 Mar 31;191(3):1081-8. doi: 10.1006/bbrc.1993.1327.
Endothelin-1 (ET-1) is a potent vasoconstrictor peptide implicated in a number of human diseases including atherosclerosis. ET-1 binds to two distinct G protein-coupled receptors, known as the ETA and ETB receptor subtypes. In this study, we have examined ET-1, ETA and ETB mRNA expression levels in human vascular cells cultured in vitro and in normal and atherosclerotic human arteries. The results indicate that (a) ET-1 mRNA is constitutively expressed by endothelial cells but not by smooth muscle cells, (b) endothelial cells express only ETB mRNA but smooth muscle cells co-express ETA and ETB mRNA, and (c) in comparison to normal aorta, ET-1 mRNA expression is elevated and endothelin receptor mRNA expression is repressed in atherosclerotic lesions.
内皮素-1（ET-1）是一种强效血管收缩肽，与包括动脉粥样硬化在内的多种人类疾病有关。ET-1与两种不同的G蛋白偶联受体结合，即ETA和ETB受体亚型。在本研究中，我们检测了体外培养的人血管细胞以及正常和动脉粥样硬化人动脉中ET-1、ETA和ETB mRNA的表达水平。结果表明：（a）ET-1 mRNA由内皮细胞组成性表达，而非平滑肌细胞；（b）内皮细胞仅表达ETB mRNA，而平滑肌细胞同时表达ETA和ETB mRNA；（c）与正常主动脉相比，动脉粥样硬化病变中ET-1 mRNA表达升高，内皮素受体mRNA表达受到抑制。
