Pagotto U, Arzberger T, Hopfner U, Weindl A, Stalla G K
Max-Planck-Institute of Psychiatry, Clinical Institute, Munich, Germany.
J Cardiovasc Pharmacol. 1995;26 Suppl 3:S104-6.
We performed in situ hybridization (ISH), using radioactively labeled deoxyoligonucleotides, to localize endothelin (ET) receptor subtype mRNAs (ETA and ETB) in a series of meningiomas in comparison to normal human meninges. Our examination also addressed the cerebellum adjacent to the meninges investigated. Tumor cells of all meningiomas examined showed strong hybridization signals for ETA receptor mRNA, whereas for ETB receptor mRNA only weak hybridization signals could be detected in endothelial cells of tumor blood vessels and in some cell clusters. mRNAs for neither ET receptor were detectable in normal leptomeninges. In human cerebellum we found ETA receptor mRNA only in large blood vessels; ETB receptor mRNA was intensely expressed in Bergmann glial cells. Neither subtype was detectable in neurons.
我们使用放射性标记的脱氧寡核苷酸进行原位杂交（ISH），以定位一系列脑膜瘤中内皮素（ET）受体亚型mRNA（ETA和ETB），并与正常人类脑膜进行比较。我们的检查还涉及所研究脑膜相邻的小脑。所有检查的脑膜瘤肿瘤细胞均显示ETA受体mRNA的强杂交信号，而对于ETB受体mRNA，仅在肿瘤血管内皮细胞和一些细胞簇中检测到弱杂交信号。在正常软脑膜中未检测到两种ET受体的mRNA。在人类小脑中，我们仅在大血管中发现了ETA受体mRNA；ETB受体mRNA在伯格曼胶质细胞中强烈表达。在神经元中未检测到任何一种亚型。
