Mathieu M N, Chevillard C
INSERM U.300, Faculté de Pharmacie, Montpellier, France.
J Cardiovasc Pharmacol. 1995;26 Suppl 3:S508-9.
Recent studies have revealed that endothelin-1 (ET-1) may be produced by human cancer cell lines and have suggested that in vivo the peptide might play a modulatory role in the growth of stromal cells surrounding tumor cells and/or in the growth of the cancer cells themselves, through paracrine or autocrine mechanisms. Therefore, we investigated whether ET-1 and ET receptors could be expressed in the human gastric cancer cell line HGT-1. By applying the reverse transcriptase polymerase chain reaction (RT-PCR) to total RNA extracted from the cells, using oligonucleotides synthesized from the sequence of the prepro-ET-1 mRNA, we have amplified a cDNA at the expected size (453 bp), which hybridized with a labeled ET-1-specific probe. In addition, RT-PCR was carried out to test whether HGT-1 cells expressed mRNA for ETA and/or ETB receptor subtypes. The amplified products of cDNA were at the size predicted for the ETA receptor (368 bp), whereas no ETB receptor mRNA could be detected.
最近的研究表明，内皮素-1（ET-1）可能由人类癌细胞系产生，并提示在体内该肽可能通过旁分泌或自分泌机制，对肿瘤细胞周围的基质细胞生长和/或癌细胞自身生长发挥调节作用。因此，我们研究了ET-1和ET受体是否能在人胃癌细胞系HGT-1中表达。通过将逆转录聚合酶链反应（RT-PCR）应用于从细胞中提取的总RNA，使用根据前体ET-1 mRNA序列合成的寡核苷酸，我们扩增出了预期大小（453 bp）的cDNA，其与标记的ET-1特异性探针杂交。此外，进行RT-PCR以检测HGT-1细胞是否表达ETA和/或ETB受体亚型的mRNA。cDNA的扩增产物大小符合ETA受体的预测大小（368 bp），而未检测到ETB受体mRNA。
