Holland D, Ben-Hayyim G, Faltin Z, Camoin L, Strosberg A D, Eshdat Y
Department of Fruit Tree Breeding and Genetics, Volcani Center, Bet Dagan, Israel.
Plant Mol Biol. 1993 Mar;21(5):923-7. doi: 10.1007/BF00027124.
A gene encoding for a citrus salt-stress-associated protein (Cit-SAP) was cloned from Citrus sinensis salt-treated cell suspension. The gene, designated csa, was isolated from a cDNA expression library. The partial amino acid sequence of the protein, as well as that deduced from the nucleotide sequence of csa, revealed a considerable homology to mammalian glutathione peroxidase (GP), and to clone 6P229 from tobacco protoplasts. The increased expression of Cit-SAP in NaCl-treated cultured citrus cells and in citrus plants irrigated with saline water, and its similarity to GP, raise the possibility that one of the effects of salt stress in plants may be the increase of the level of free radicals.
从盐处理的甜橙细胞悬浮液中克隆出一个编码柑橘盐胁迫相关蛋白（Cit-SAP）的基因。该基因命名为csa，是从一个cDNA表达文库中分离得到的。该蛋白的部分氨基酸序列以及从csa核苷酸序列推导出来的氨基酸序列，显示出与哺乳动物谷胱甘肽过氧化物酶（GP）以及烟草原生质体中的克隆6P229有相当高的同源性。在经NaCl处理的培养柑橘细胞和用盐水灌溉的柑橘植株中，Cit-SAP的表达增加，以及它与GP的相似性，增加了植物盐胁迫的影响之一可能是自由基水平升高的可能性。
