磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPX）在感知 NaCl 胁迫中的信号作用，伴随黄化向日葵幼苗子叶的出现。
Signaling role of phospholipid hydroperoxide glutathione peroxidase (PHGPX) accompanying sensing of NaCl stress in etiolated sunflower seedling cotyledons.
机构信息
a Laboratory of Plant Physiology and Biochemistry; Department of Botany; University of Delhi ; Delhi , India.
出版信息
Plant Signal Behav. 2014;9(12):e977746. doi: 10.4161/15592324.2014.977746.
Abstract
Sunflower seedlings subjected to 120 mM NaCl stress exhibit high total peroxidase activity, differential expression of its isoforms and accumulation of lipid hydroperoxides. This coincides with high specific activity of phospholipid hydroperoxide glutathione peroxidase (PHGPX) in the 10,000g supernatant from the homogenates of 2-6 d old seedling cotyledons. An upregulation of PHGPX activity by NaCl is evident from Western blot analysis. Confocal laser scanning microscopic (CLSM) analysis of sections of cotyledons incubated with anti-GPX4 (PHGPX) antibody highlights an enhanced cytosolic accumulation of PHGPX, particularly around the secretory canals. Present work, thus, highlights sensing of NaCl stress in sunflower seedlings in relation with lipid hydroperoxide accumulation and its scavenging through an upregulation of PHGPX activity in the cotyledons.
摘要
向日葵幼苗在遭受 120mM NaCl 胁迫时表现出高总过氧化物酶活性、同工型的差异表达和脂质氢过氧化物的积累。这与来自 2-6 天大幼苗子叶匀浆的 10000g 上清液中磷脂氢过氧化物谷胱甘肽过氧化物酶 (PHGPX) 的高比活性相一致。Western blot 分析表明 NaCl 对 PHGPX 活性的上调。用抗 GPX4（PHGPX）抗体孵育子叶切片的共焦激光扫描显微镜 (CLSM) 分析突出显示 PHGPX 在细胞质中的积累增加，特别是在分泌道周围。因此，本研究强调了向日葵幼苗在与脂质氢过氧化物积累有关的 NaCl 胁迫感应，以及通过在子叶中上调 PHGPX 活性来清除脂质氢过氧化物。
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