鉴定和纯化牛弓首蛔虫特异性抗原以改善血清学诊断。

Identification and purification of Cooperia oncophora-specific antigens to improve serological diagnosis.

作者信息

de Graaf D C, Berghen P, Hilderson H, De Cock H, Vercruysse J

机构信息

Department of Parasitology, Faculty of Veterinary Medicine, University of Gent, Belgium.

出版信息

Int J Parasitol. 1993 Feb;23(1):141-4. doi: 10.1016/0020-7519(93)90108-b.

DOI:10.1016/0020-7519(93)90108-b

PMID:8468130

Abstract

Cooperia oncophora total adult extracts were examined by Western blotting with sera from C. oncophora- and O. ostertagi-infected calves to determine species-specific antigens. It was shown that two antigens with apparent molecular weights of 14.2 and 14.9 kDa were only recognized by calves which received a Cooperia infection and not by Ostertagia mono-infected calves or parasite-naive animals. The partial purification of these two antigens was achieved by gel filtration and ion-exchange chromatography. An enzyme-linked immunosorbent assay (ELISA) was developed based on the fractions containing these two antigens and no cross-reactivity could be noticed with serum from Ostertagia mono-infected calves. In contrast, the ELISA with total worm extracts showed strong cross-reactivity with heterologous serum. It was concluded that the 14.2 and 14.9 kDa Cooperia adult antigens have diagnostic potential, at least to differentiate C. oncophora and O. ostertagi.

摘要

利用感染了柯氏类圆线虫（Cooperia oncophora）和奥氏奥斯特线虫（Ostertagia ostertagi）的犊牛血清，通过蛋白质免疫印迹法检测柯氏类圆线虫成虫的总提取物，以确定种特异性抗原。结果表明，表观分子量为14.2 kDa和14.9 kDa的两种抗原仅被感染柯氏类圆线虫的犊牛识别，而未被单一感染奥氏奥斯特线虫的犊牛或未接触过寄生虫的动物识别。通过凝胶过滤和离子交换色谱法对这两种抗原进行了部分纯化。基于含有这两种抗原的组分开发了一种酶联免疫吸附测定（ELISA），未发现与单一感染奥氏奥斯特线虫的犊牛血清有交叉反应。相比之下，用虫体总提取物进行的ELISA显示与异源血清有强烈的交叉反应。得出的结论是，14.2 kDa和14.9 kDa的柯氏类圆线虫成虫抗原具有诊断潜力，至少可用于区分柯氏类圆线虫和奥氏奥斯特线虫。