Santarén J F, Alconada A, Cuezva J M
Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
J Biochem. 1993 Feb;113(2):129-31. doi: 10.1093/oxfordjournals.jbchem.a124014.
We report the one-step processing of the rat liver beta-F1-ATPase precursor protein, as examined by high resolution 2D-gel electrophoresis. Proteolytic cleavage of the positively charged mitochondrial targeting signal of the precursor promotes decreases in both the molecular weight (approximately 3 kDa) and the isoelectric point (approximate 0.2 pH unit) of the protein. The results obtained illustrate the usefulness of this technique, since it takes advantage of both results of the maturation process, for molecular characterization of the processing of mitochondrial precursor proteins.
我们报告了通过高分辨率二维凝胶电泳检测大鼠肝脏β-F1-ATP酶前体蛋白的一步加工过程。前体蛋白带正电的线粒体靶向信号的蛋白水解切割促进了该蛋白分子量（约3 kDa）和等电点（约0.2个pH单位）的降低。所获得的结果说明了该技术的实用性，因为它利用了成熟过程的两个结果，用于线粒体前体蛋白加工的分子表征。
