Haslam S Z, Counterman L J, Nummy K A
Physiology Department, Michigan State University, East Lansing 48823-1101.
J Cell Physiol. 1993 Apr;155(1):72-8. doi: 10.1002/jcp.1041550110.
Estrogen (E), progesterone (P), and epidermal growth factor (EGF) are involved in the growth and development of the normal mammary gland. While studies have been carried out to investigate the in vivo effects of EGF in the immature mammary gland, nothing is known about the growth effects of EGF or its potential interactions with E and/or P in the adult mammary gland. The present studies were undertaken to investigate the effects of EGF, E, and P on mammary cell proliferation in immature, peripubertal vs. adult, sexually mature mice. We have found that EGF promotes epithelial and stromal cell proliferation in both the immature and adult mammary glands. In the immature gland, the end bud epithelium is most responsive to the proliferative effects of EGF and there is no apparent interaction between EGF, E, and/or P. In contrast, in the mature gland EGF adds to the proliferative effects of E+P in the ductal epithelium resulting in more extensive ductal sidebranching. Thus these results demonstrate that the developmental state of the mammary gland determines the nature and extent of the interactions between EGF, E, and P in growth and development.
雌激素（E）、孕激素（P）和表皮生长因子（EGF）参与正常乳腺的生长和发育。虽然已经开展了研究来调查EGF在未成熟乳腺中的体内作用，但关于EGF在成年乳腺中的生长作用或其与E和/或P的潜在相互作用却一无所知。本研究旨在调查EGF、E和P对未成熟、青春期前后与成年、性成熟小鼠乳腺细胞增殖的影响。我们发现，EGF可促进未成熟和成年乳腺中的上皮细胞和基质细胞增殖。在未成熟乳腺中，终末芽上皮对EGF的增殖作用最为敏感，且EGF、E和/或P之间没有明显的相互作用。相比之下，在成熟乳腺中，EGF增强了E+P对导管上皮的增殖作用，导致更广泛的导管侧支形成。因此，这些结果表明，乳腺的发育状态决定了EGF、E和P在生长发育过程中相互作用的性质和程度。
