Haslam S Z, Counterman L J, Nummy K A
Physiology Department, Michigan State University, East Lansing 48823-1101.
J Cell Physiol. 1992 Sep;152(3):553-7. doi: 10.1002/jcp.1041520315.
Estrogen (E), progesterone (P), and epidermal growth factor (EGF) are known to regulate growth and development of the normal mammary gland, and it is possible that EGF may interact with E and/or P. Estrogen (ER), progesterone (PR), and EGF receptors (EGF-R) have been detected in both mammary epithelial and stromal cells, and the relative roles of the various cells types in hormone-dependent growth regulation are not known. The present studies were undertaken to determine if E and/or P influence EGF action by exerting a regulatory effect on EGF-R levels and which cell types are affected. The comparative effects of ovariectomy and hormone treatments on EGF-R levels were examined in immature, pubertal 5-week-old and sexually mature 10-week-old female mice. EGF-R were characterized as a single class of high affinity sites and EGF-R concentration was 2-fold higher in glands of 5-week-old mice. Ovariectomy had no significant effect on EGF-R concentration in either age group, and treatment with E and/or P had no effect on EGF-R levels in either epithelial or stromal cells in 5-week-old mice. In contrast, E+P treatment caused a 2-fold increase in receptor concentration in 10-week-old mice in the mammary epithelium. Thus it appears that the developmental state of the gland may determine the nature and extent of the interaction of of EGF, E, and P.
已知雌激素（E）、孕激素（P）和表皮生长因子（EGF）可调节正常乳腺的生长和发育，并且EGF可能与E和/或P相互作用。在乳腺上皮细胞和基质细胞中均已检测到雌激素受体（ER）、孕激素受体（PR）和EGF受体（EGF-R），但不同细胞类型在激素依赖性生长调节中的相对作用尚不清楚。本研究旨在确定E和/或P是否通过对EGF-R水平发挥调节作用来影响EGF的作用，以及哪些细胞类型会受到影响。在未成熟、5周龄青春期和10周龄性成熟的雌性小鼠中，研究了卵巢切除术和激素处理对EGF-R水平的比较影响。EGF-R被表征为单一类别的高亲和力位点，5周龄小鼠腺体中的EGF-R浓度高2倍。卵巢切除术对两个年龄组的EGF-R浓度均无显著影响，E和/或P处理对5周龄小鼠上皮细胞或基质细胞中的EGF-R水平均无影响。相比之下，E+P处理使10周龄小鼠乳腺上皮中的受体浓度增加了2倍。因此，似乎腺体的发育状态可能决定EGF、E和P相互作用的性质和程度。
