Morooka T, Shibata M, Yasumoto S, Takeo H, Oda T, Murakami N, Hirose M, Kai A, Kudoh Y
Department of Pediatrics, Fukuoka University.
Kansenshogaku Zasshi. 1993 Feb;67(2):122-6. doi: 10.11150/kansenshogakuzasshi1970.67.122.
We tried to isolate verotoxin-producing Escherichia coli (VTEC) from 323 sporadic pediatric enteritis patients who came to three clinics in the Fukuoka area. We used the sorbitol-MacConkey medium for the isolation of VTEC O157:H7. For non-O157 VTEC strains we used the V1/PECS method. VT/PECS method was applied. VTEC strains were isolated from three patients (0.9%). None of the patients were seriously, ill or developed the hemolytic uremic syndrome. The three patients were all seen in the summer season, July and August. O157:H7 strains were isolated from two patients, and O145:NM from one. This study showed that sporadic enteritis cases due to VTEC exist in the Fukuoka area. In the future a rapid and easy method for the detection of VT or VTEC should be developed and commercialized to proceed with epidemiological studies of VTEC infections throughout Japan.
我们试图从323名前来福冈地区三家诊所就诊的散发性小儿肠炎患者中分离出产生志贺毒素的大肠杆菌(VTEC)。我们使用山梨醇麦康凯培养基分离VTEC O157:H7。对于非O157 VTEC菌株,我们使用V1/PECS方法。应用了VT/PECS方法。从三名患者(0.9%)中分离出VTEC菌株。所有患者均未出现严重疾病或发展为溶血尿毒综合征。这三名患者均在夏季的7月和8月就诊。从两名患者中分离出O157:H7菌株,从一名患者中分离出O145:NM。本研究表明,福冈地区存在由VTEC引起的散发性肠炎病例。未来应开发一种快速简便的VT或VTEC检测方法并实现商业化,以便在日本全国开展VTEC感染的流行病学研究。