Bielaszewska M, Janda J, Blahová K, Feber J, Potuzník V, Soucková A
Institute of Medical Microbiology, University Hospital Motol, Prague, Czech Republic.
Clin Nephrol. 1996 Jul;46(1):42-4.
Since October 1988 till July 1995, 35 children (mean age 16 +/- 15.5 months) with classical hemolytic uremic syndrome (HUS) were examined for the presence of verocytotoxin (VT)-producing Escherichia coli (VTEC) infection. Stool samples from all patients were cultured for VTEC strains and tested for free fecal neutralizable VT. Serum samples from 18 patients taken on admission were also tested for antibodies to the lipopolysaccharide (LPS) from E. coli O157, O26, O55, O111, and O128 using the passive hemagglutination assay (PHA). Diagnosis of VTEC infection was established in 28 (80%) patients by the combined use of microbiological and serological techniques. VTEC were isolated from 16 (46%) patients, 5 of them had infection with 2 different VTEC serotypes; 11 (52%) VTEC isolates belonged to the serotypes O26: H11 (5), and O157: H7/NM (6). Free fecal VT was found in 21 (60%) patients. PHA antibodies to one or more LPS were detected in 14 (78%) of 18 patients; 9 had antibodies to O157 LPS and 6 to O26 LPS. We conclude from this study that VTEC are the important cause of pediatric HUS in the Czech Republic and the strains belonging to the serogroups O157 and O26 are the most prevalent.
自1988年10月至1995年7月，对35例（平均年龄16±15.5个月）典型溶血尿毒综合征（HUS）患儿进行了产志贺毒素（VT）大肠杆菌（VTEC）感染检测。对所有患者的粪便样本进行VTEC菌株培养，并检测游离粪便中可中和的VT。还采用被动血凝试验（PHA）对18例入院患者的血清样本检测针对大肠杆菌O157、O26、O55、O111和O128脂多糖（LPS）的抗体。通过微生物学和血清学技术联合检测，确诊28例（80%）患者存在VTEC感染。16例（46%）患者分离出VTEC，其中5例感染2种不同的VTEC血清型；11株（52%）VTEC分离株属于O26:H11血清型（5株）和O157:H7/NM血清型（6株）。21例（60%）患者粪便中检测到游离VT。18例患者中有14例（78%）检测到针对一种或多种LPS的PHA抗体；9例有针对O157 LPS的抗体，6例有针对O26 LPS的抗体。我们从这项研究得出结论，VTEC是捷克共和国小儿HUS的重要病因，O157和O26血清群的菌株最为常见。
