Sambri V, Basso F, Massaria F, Ardizzoni M, Cevenini R
Institute of Microbiology, University of Bologna, St. Orsola Hospital, Italy.
New Microbiol. 1993 Jan;16(1):43-9.
This study investigated the ability of Borrelia burgdorferi and Borrelia hermsii to attach the surface of several types of in vitro-cultured mammalian cells. Borreliae showed different adhesion efficiencies depending on cell type and temperature. Temperatures both lower and higher than 33 degrees and 37 degrees C respectively, decreased the adhesion of borreliae which preferentially adhere to human fibroblast-like cells. The adhesion process, mediated by structures exposed onto the surface of the microorganisms, also proved to be sensitive to the treatment of mammalian cells with hyaluronidase and sialidase, confirming that carbohydrate receptors are involved in the adhesion of borreliae to eukaryotic cells.
本研究调查了伯氏疏螺旋体和赫氏疏螺旋体附着于几种体外培养的哺乳动物细胞表面的能力。疏螺旋体根据细胞类型和温度表现出不同的黏附效率。分别低于33摄氏度和高于37摄氏度的温度会降低疏螺旋体的黏附，而疏螺旋体优先黏附于人类成纤维细胞样细胞。由微生物表面暴露的结构介导的黏附过程，也被证明对用透明质酸酶和唾液酸酶处理哺乳动物细胞敏感，这证实了碳水化合物受体参与了疏螺旋体与真核细胞的黏附。
