Steel M S, Naughton J M, Hopkins G W, Sinclair A J, O'Dea K
University of Melbourne, Department of Medicine, Royal Melbourne Hospital, Parkville, Victoria, Australia.
Prostaglandins Leukot Essent Fatty Acids. 1993 Mar;48(3):247-51. doi: 10.1016/0952-3278(93)90093-c.
Male Sprague Dawley rats were fed a butter-enriched diet (50% fat) for 2 weeks which was supplemented orally with 9, 18, 36, or 72 mg/day of ethyl arachidonate for a further 2 weeks. The control group of animals were fed a 5% fat diet for 4 weeks. Aortic prostacyclin (PGI2) production, platelet aggregation and thromboxane A2 (TXA2) production and plasma and aortic phospholipid (PL) fatty acids were measured. 50% butter-feeding resulted in a significant reduction in aortic PGI2 production and collagen-induced platelet aggregation and TXA2 production. These changes were accompanied by a reduction in plasma and aortic PL arachidonic acid levels and an increase in eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), 5,8,11-eicosatrienoic acid (ETA) and dihomo-gamma-linolenic acid (DGLA). These changes in prostanoid production, platelet aggregation and PL fatty acid composition were dose-dependently reversed by the daily oral administration of ethyl arachidonate (9, 18, 36, or 72 mg). The threshold dose being as little as 9 mg of ethyl arachidonate/rat/day for reversal of PL fatty acid composition, collagen-induced platelet aggregation and TXA2 production, and 18 mg of ethyl arachidonate/rat/day for reversal of aortic PGI2 production. Full reversal was seen generally with 36 or 72 mg of ethyl arachidonate/rat/day. The data highlight the responsiveness of tissue eicosanoid production to small quantities (ppm) of dietary eicosanoid precursors.
将雄性斯普拉格-道利大鼠喂食富含黄油的饮食（脂肪含量50%）2周，随后再口服补充9、18、36或72毫克/天的花生四烯酸乙酯，持续2周。对照组动物喂食脂肪含量5%的饮食4周。检测主动脉前列环素（PGI2）生成、血小板聚集、血栓素A2（TXA2）生成以及血浆和主动脉磷脂（PL）脂肪酸情况。喂食50%黄油导致主动脉PGI2生成、胶原诱导的血小板聚集及TXA2生成显著降低。这些变化伴随着血浆和主动脉PL花生四烯酸水平降低以及二十碳五烯酸（EPA）、二十二碳六烯酸（DHA）、5,8,11-二十碳三烯酸（ETA）和二高-γ-亚麻酸（DGLA）增加。通过每日口服花生四烯酸乙酯（9、18、36或72毫克），这些前列腺素生成、血小板聚集和PL脂肪酸组成的变化呈剂量依赖性逆转。逆转PL脂肪酸组成、胶原诱导的血小板聚集和TXA2生成的阈值剂量低至9毫克花生四烯酸乙酯/大鼠/天，逆转主动脉PGI2生成的阈值剂量为18毫克花生四烯酸乙酯/大鼠/天。一般给予36或72毫克花生四烯酸乙酯/大鼠/天可实现完全逆转。数据突出了组织类花生酸生成对少量（ppm）膳食类花生酸前体的反应性。
