Pedraza-Chaverrí J, Calderón P, Cruz C, Peña J C
Departamento de Nefrología y Metabolismo Mineral, Instituto Nacional de la Nutrición Salvador Zubirán, México, DF.
Ren Fail. 1993;15(2):149-55. doi: 10.3109/08860229309046146.
Albumin; and alpha 1-, alpha 2-, beta-, and gamma-globulins were estimated by cellulose acetate electrophoresis in the serum and urine from rats with nephrotic syndrome (NS), 2, 4, 6, 8, 10, 12, 16, 20, and 30 days after a single injection of puromycin aminonucleoside (PAN). It was found that: (a) total serum protein level decreased on days 4-16, and total urine protein excretion rose on days 6-16; (b) serum albumin level fell on days 4-16, and urine albumin excretion increased on days 6-16; (c) serum alpha 1-globulin level rose on days 8-30, and urine alpha 1-globulin excretion increased on days 8-16; (d) serum alpha 2-globulin level remained essentially unchanged, and urine alpha 2-globulin excretion rose on days 4-10; (e) serum beta-globulin level decreased on days 4-20, and urine beta-globulin excretion increased on days 6-16, (f) serum gamma-globulin level diminished on days 6, 8, and 12, and urine gamma-globulin excretion rose on days 6-10. All serum protein fractions were excreted in the urine of nephrotic rats; these findings suggest that proteinuria is nonselective. The differences observed in the serum protein profiles, even when all protein fractions were lost in the urine, suggest an independent regulation of each protein fraction in PAN-nephrotic rats. In addition, the electrophoretic profile of serum proteins in PAN-nephrotic rats is different from previously reported patterns in human nephrosis and in rats with an acute-phase response.
通过醋酸纤维素电泳法对单次注射嘌呤霉素氨基核苷（PAN）后2、4、6、8、10、12、16、20和30天的肾病综合征（NS）大鼠血清和尿液中的白蛋白以及α1-、α2-、β-和γ-球蛋白进行了测定。结果发现：（a）血清总蛋白水平在第4 - 16天下降，尿总蛋白排泄量在第6 - 16天上升；（b）血清白蛋白水平在第4 - 16天下降，尿白蛋白排泄量在第6 - 16天增加；（c）血清α1-球蛋白水平在第8 - 30天上升，尿α1-球蛋白排泄量在第8 - 16天增加；（d）血清α2-球蛋白水平基本保持不变，尿α2-球蛋白排泄量在第4 - 10天上升；（e）血清β-球蛋白水平在第4 - 20天下降，尿β-球蛋白排泄量在第
