Yang P M, Olee T, Carson D A, Chen P P
Department of Medicine, University of California, San Diego, La Jolla 92093-0663.
Scand J Immunol. 1993 Apr;37(4):504-8. doi: 10.1111/j.1365-3083.1993.tb03326.x.
Previously, we showed that the Humha 1lr rearranged gene was almost identical to the consensus amino acid sequence of several G6 idiotype-positive rheumatoid factor (RF) heavy chains, and to the VH gene-encoded region of the fetally expressed 51P1 cDNA. The finding led us to suggest that the ha 1lr-corresponding germline gene encodes the heavy chains of many human IgM RFs. We now report the isolation of the proposed germline gene, designated Humhv1051; it is identical to the consensus sequence of the G6 heavy chain V regions. During this experiment, we also isolated unexpectedly an additional VH1 gene, termed Humhv1051K; it differs from hv1051 by one amino acid residue. Importantly, hv1051K is identical to Humha113, the rearranged VH1 gene of a natural anti-cardiolipin antibody.
此前，我们发现Humha 1lr重排基因与几种G6独特型阳性类风湿因子（RF）重链的共有氨基酸序列几乎相同，且与胎儿表达的51P1 cDNA的VH基因编码区相同。这一发现使我们推测，与ha 1lr对应的种系基因编码许多人IgM RF的重链。我们现在报告了拟种系基因的分离，命名为Humhv1051；它与G6重链V区的共有序列相同。在这个实验中，我们还意外地分离出了另一个VH1基因，称为Humhv1051K；它与hv1051有一个氨基酸残基的差异。重要的是，hv1051K与天然抗心磷脂抗体的重排VH1基因Humha113相同。
