Alwan W H, Openshaw P J
Department of Medicine, St Mary's Hospital Medical School, London, UK.
Vaccine. 1993;11(4):431-7. doi: 10.1016/0264-410x(93)90284-5.
Mice were infected with respiratory syncytial (RS) virus or with recombinant vaccinia viruses (rVV) expressing individual RS virus proteins. rVV-G, rVV-F and, to a lesser extent, rVV-P induced ELISA-binding anti-RS virus antibodies; those induced by rVV-P were non-neutralizing. Different antigens induced helper T cells with distinct cytokine secretion profiles: some released IL-2, and others predominantly IL-4 and 5. Virus-specific cytotoxicity was induced by infection with RS virus, rVV-F or rVV-22K. Different RS virus proteins (given in the same route and form) therefore prime for functionally distinct T-cell activities. These patterns of virus-specific immunity may help explain the pathogenicity of RS virus vaccines, and help in the design of protective, non-pathogenic vaccines in the future.
将小鼠感染呼吸道合胞（RS）病毒或表达单个RS病毒蛋白的重组痘苗病毒（rVV）。rVV-G、rVV-F以及程度稍轻的rVV-P可诱导ELISA结合抗RS病毒抗体；rVV-P诱导产生的抗体无中和作用。不同抗原诱导出具有不同细胞因子分泌谱的辅助性T细胞：一些释放白细胞介素-2（IL-2），另一些则主要释放IL-4和IL-5。感染RS病毒、rVV-F或rVV-22K可诱导病毒特异性细胞毒性。因此，不同的RS病毒蛋白（以相同途径和形式给予）引发功能不同的T细胞活性。这些病毒特异性免疫模式可能有助于解释RS病毒疫苗的致病性，并有助于未来设计出具有保护性的非致病性疫苗。
