肽抗生素枯草菌素的一种新型翻译后修饰：从枯草芽孢杆菌美国模式培养物保藏中心6633中分离并鉴定一种天然变体
A novel post-translational modification of the peptide antibiotic subtilin: isolation and characterization of a natural variant from Bacillus subtilis A.T.C.C. 6633.
作者信息
Chan W C, Bycroft B W, Leyland M L, Lian L Y, Roberts G C
机构信息
Department of Pharmaceutical Sciences, University of Nottingham, U.K.
出版信息
Biochem J. 1993 Apr 1;291 ( Pt 1)(Pt 1):23-7. doi: 10.1042/bj2910023.
Abstract
A variant of the peptide antibiotic subtilin has been isolated from Bacillus subtilis A.T.C.C. 6633, and its structure has been shown to be [N alpha-succinyl-Trp1]subtilin. The chemical structure of a fragment derived by tryptic hydrolysis of the variant is shown to be N alpha-succinyl-Trp-Lys by 1H and 13C n.m.r., fast-atom-bombardment m.s. and total chemical synthesis [N alpha-Succinyl-Trp1]-subtilin is produced later in the growth of the bacterium than is subtilin; reverse-phase h.p.l.c. analysis shows that after 24 h growth the ratio subtilin/[N alpha-succinyl-Trp1]subtilin is approx. 1:2. Although [N alpha-succinyl-Trp1]subtilin retains significant antibacterial activity, it is 10-20 times less active than subtilin.
摘要
一种肽抗生素枯草菌素的变体已从枯草芽孢杆菌美国典型培养物保藏中心6633菌株中分离出来，其结构已被证明为[Nα-琥珀酰-色氨酸1]枯草菌素。通过1H和13C核磁共振、快原子轰击质谱和全化学合成表明，该变体经胰蛋白酶水解得到的一个片段的化学结构为Nα-琥珀酰-色氨酸-赖氨酸。[Nα-琥珀酰-色氨酸1]枯草菌素在细菌生长后期产生，比枯草菌素产生得晚；反相高效液相色谱分析表明，生长24小时后，枯草菌素/[Nα-琥珀酰-色氨酸1]枯草菌素的比例约为1:2。虽然[Nα-琥珀酰-色氨酸1]枯草菌素保留了显著的抗菌活性，但其活性比枯草菌素低10至20倍。
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