Earnest D L, Abril E R, Jolley C S, Martinez F
Department of Medicine, University of Arizona College of Medicine, Tucson 85724.
Alcohol Alcohol. 1993 Jan;28(1):73-83.
The effects of 6 weeks of alcohol feeding on phagocytic, metabolic and secretory functions as well as gene expression of hepatic Kupffer cells were evaluated in vitro using cultured Kupffer cells isolated from male Sprague-Dawley rats. The rats were fed either Teklad pelleted rat chow or the 1982 Lieber-DeCarli liquid diet containing 6% ethanol (36% calories) or the same liquid diet with maltose-dextrin isocalorically substituted for the alcohol. Weight gain was greatest in the chow-fed animals and least in those receiving ethanol. The alcohol-containing diet stimulated Kupffer cell phagocytosis, mitochondrial reduction of MTT, secretion of tumor necrosis factor (TNF) and expression of TNF mRNA. However, each of these cell functions was also enhanced by the control Lieber-DeCarli liquid diet alone and the stimulating effect of the control diet often exceeded that induced by ethanol. The results suggest that early in chronic alcohol consumption, the immune system may be stimulated by ethanol, and that during studies of ethanol-induced changes in immune system function, close attention must be given to potentially confounding effects of the diet.
使用从雄性斯普拉格 - 道利大鼠分离的培养库普弗细胞，在体外评估了6周酒精喂养对肝库普弗细胞吞噬、代谢和分泌功能以及基因表达的影响。给大鼠喂食Teklad颗粒大鼠饲料，或含有6%乙醇（36%卡路里）的1982年Lieber - DeCarli液体饮食，或用麦芽糖糊精等热量替代酒精的相同液体饮食。体重增加在喂食饲料的动物中最大，在接受乙醇的动物中最小。含酒精饮食刺激了库普弗细胞吞噬作用、MTT的线粒体还原、肿瘤坏死因子（TNF）分泌和TNF mRNA表达。然而，这些细胞功能中的每一项也被单独的对照Lieber - DeCarli液体饮食增强，并且对照饮食的刺激作用通常超过乙醇诱导的作用。结果表明，在慢性酒精消费早期，免疫系统可能受到乙醇刺激，并且在乙醇诱导的免疫系统功能变化研究期间，必须密切关注饮食的潜在混杂效应。
