Sampson H W, Hebert V A, Booe H L, Champney T H
Department of Human Anatomy and Neurobiology, College of Medicine, Texas A&M University Health Science Center, College Station 77843-1114, USA.
Alcohol Clin Exp Res. 1998 Nov;22(8):1746-53.
To determine the effect of life-long alcohol consumption on the adult and aged rat model, 4-week-old, female Sprague-Dawley rats were divided into three diet groups. Alcohol-treated animals were fed a modified Lieber-DeCarli diet ad libitum containing 35% ethanol-derived calories, whereas the pair-fed animals (weight-matched to ethanol rats) received an isocaloric liquid diet in which maltose-dextrin substituted calories supplied by ethanol. Chow animals were fed a standard rat chow ad libitum. Proximal tibiae (primarily cancellous bone) and femora (primarily cortical bone) were removed for analysis after 3, 6, 9, 12, or 18 months on the diets. Serum was collected for analysis of calcium levels, the calcium regulating hormones; parathyroid hormone, 25-hydroxyvitamin D, calcitonin, corticosterone, estradiol, testosterone, and IGF-1. Creatinine, SGOT/AST, and SGPT/ALT levels were measured to determine kidney and liver integrity. Previous studies, with young animals, showed that chronic alcohol consumption during the age of bone development reduced bone density and bone mass in both cortical and cancellous bone. The present study demonstrates that these reductions last throughout life, whereas morphological values, such as length and diameter, attain control levels. Calcium regulating hormones and sex hormones are essentially normal and do not appear to be the primary causative agent for adult alcohol-induced osteopenia, but it appears to be due to a more direct effect of alcohol on bone cells.
为了确定终生饮酒对成年和老年大鼠模型的影响，将4周龄的雌性斯普拉格-道利大鼠分为三个饮食组。给予酒精处理的动物随意进食改良的Lieber-DeCarli饮食，其中含35%乙醇衍生热量，而配对喂养的动物（体重与乙醇喂养大鼠匹配）接受等热量液体饮食，其中麦芽糖糊精替代了乙醇提供的热量。给予普通饮食的动物随意进食标准大鼠饲料。在饮食3、6、9、12或18个月后，取出近端胫骨（主要为松质骨）和股骨（主要为皮质骨）进行分析。收集血清以分析钙水平、钙调节激素；甲状旁腺激素、25-羟基维生素D、降钙素、皮质酮、雌二醇、睾酮和胰岛素样生长因子-1。测量肌酐、谷草转氨酶/天冬氨酸转氨酶和谷丙转氨酶/丙氨酸转氨酶水平以确定肾脏和肝脏的完整性。先前对幼龄动物的研究表明，在骨骼发育年龄期间长期饮酒会降低皮质骨和松质骨的骨密度和骨量。本研究表明，这些降低会持续终生，而诸如长度和直径等形态学值则达到对照水平。钙调节激素和性激素基本正常，似乎不是成年期酒精性骨质减少的主要致病因素，但似乎是由于酒精对骨细胞的更直接作用。
