Humphrey R L, Trivedi S M, Frondoza C G
Cell Tissue Kinet. 1979 Jan;12(1):71-80. doi: 10.1111/j.1365-2184.1979.tb00114.x.
[16-14C]Arginine ([6-14C]Arg) was used as an in vivo pulse label to study BALB/c murine LPC-1 plasmacytoma synthesis and secretion of its tumour-associated M component (IgG2a, kappa). With this isotope, an eight- to ten-fold enhancement in the labelling of the gamma globulin region and ten-fold reduction in the albumin labelling were observed. Production and secretion of the M component was detected (within 30 min) after cell transfer. Only mice which received tumour cells showed significant labelling in the gamma globulin region 24 hr after isotope injection. The labelling behaviour of the tumour M component correlated with the administered cell dose. The peak heights of radioactivity in the gamma region increased with increments in cell number. When the percentage radioactivity diverted into M component was plotted as a function of cell dose, a linear relationship was noted. This study demonstrates the feasibility of using [6-14C]Arg as a tool to follow the newly synthesized tumour-associated protein, and provides a means of estimating tumour cell number.
[16-14C]精氨酸（[6-14C]Arg）被用作体内脉冲标记物，以研究BALB/c小鼠LPC-1浆细胞瘤合成和分泌其肿瘤相关M成分（IgG2a，κ）的情况。使用这种同位素时，观察到γ球蛋白区域的标记增强了8至10倍，白蛋白标记减少了10倍。细胞转移后（30分钟内）检测到了M成分的产生和分泌。同位素注射24小时后，只有接受肿瘤细胞的小鼠在γ球蛋白区域显示出明显的标记。肿瘤M成分的标记行为与所给予的细胞剂量相关。γ区域放射性的峰值高度随着细胞数量的增加而增加。当将转入M成分的放射性百分比作为细胞剂量的函数绘制时，发现呈线性关系。本研究证明了使用[6-14C]Arg作为追踪新合成的肿瘤相关蛋白的工具的可行性，并提供了一种估计肿瘤细胞数量的方法。
