McGuckin M A, Ramm L E, Joy G J, Devine P L, Ward B G
Department of Obstetrics and Gynaecology, University of Queensland, Herston, Australia.
Clin Chim Acta. 1993 Feb 28;214(2):139-51. doi: 10.1016/0009-8981(93)90106-e.
This investigation was undertaken to establish a reference range for tumour-associated MUC1 mucins in the serum of healthy women of the ages at risk for adenocarcinoma of the ovary and breast. Blood samples and clinical information were obtained from 5,000 women attending a breast screening mammography clinic. Data from women diagnosed with breast carcinoma and those subsequently diagnosed with other cancers were omitted from the reference range. Mucin concentrations were measured using the CASA assay which detects the protein core of MUC1 encoded mucins. Multiple linear regression analysis showed no effect on CASA concentrations by non-malignant changes to the breast, menopausal status, presence/absence of the reproductive tract, parity or history of hormone use. However, CASA concentrations were significantly increased in smokers (P < 0.001) and progressively increased with age (P < 0.001). These data show that these factors must be given consideration when setting upper limits of normal using MUC1 protein core binding assays.
本研究旨在为处于卵巢癌和乳腺癌发病风险年龄的健康女性血清中的肿瘤相关MUC1粘蛋白建立一个参考范围。从一家乳腺筛查钼靶诊所的5000名女性中获取了血液样本和临床信息。被诊断为乳腺癌以及随后被诊断为其他癌症的女性的数据被排除在参考范围之外。使用CASA检测法测量粘蛋白浓度，该方法可检测MUC1编码粘蛋白的蛋白核心。多元线性回归分析表明，乳腺的非恶性变化、绝经状态、生殖道的有无、生育情况或激素使用史对CASA浓度没有影响。然而，吸烟者的CASA浓度显著升高（P < 0.001），并且随着年龄的增长逐渐升高（P < 0.001）。这些数据表明，在使用MUC1蛋白核心结合检测法设定正常上限时，必须考虑这些因素。
